Yasmina H. Debbab
BIOL 3810-502
LAB REPORT #1
COMPOUND ACTION POTENTIAL (5)
DATE PERFORMED: February 13th, 2015
LAB PARTNERS: #1-3
TA: JAMES CUMMINS
Introduction The sciatic nerve in the double pithed bullfrog is made up of a large bundle of various nerve fibers. Nerve fibers are defined by Eckert as “elongated cylindrical processes of a neuron along which action potentials are conducted” (G15, Eckert, 2001) and are vital to the successful conduction of this experiment. By stimulating the frog’s sciatic nerve with recording/stimulating electrodes in the nerve bath, students were able to observe the resultant electrical activity that occurred (CAP) through the PowerLab software. An understanding of neural anatomy is vital to maintain …show more content…
The absolute refractory period occurs either during or after the action potential when an electrical stimulus is completely unable to produce an excitation response in the neuron (nerve cell) no matter the strength of that second stimulus. The refractory period is the period of time following excitation that is necessary for a cell membrane to recover and be ready for a second electrical stimulus after it has returned to its original resting state (Curran, 2014). Relative refractory period is the period that according to the McGill Lab “immediately follows the absolute refractory period” when the action potential is unable to be initiated unless it is exposed to a stronger than average electrical stimulus (McGill, 2014). The refractory periods experiment involved the stimulation of the sciatic nerve at various intervals that progressively decreased by .5 ms each time before a new run was recorded. The end of the refractory period was marked by making a notation at which stimulus interval indicated a decrease in the magnitude of the second …show more content…
K., Ransom, B. R., & Waxman, S. G. (1991). Compound action potential of nerve recorded by suction electrode: a theoretical and experimental analysis. Brain research, 546(1), 18-32.
• DEPARTMENT, B. (2014, February 4). COMPOUND ACTION POTENTIAL: NERVE CONDUCTION. Retrieved March 7, 2015, from http://www.biopac.com/curriculum/pdf/a03.pdf
• Dalkilic, N., & Pehlivan, F. (1994). Derivatives and integrals of compound action potential of isolated frog sciatic nerves. J Ankara Med School, 16, 1147-1155.
• Mizuta, K., Fujita, T., Nakatsuka, T., & Kumamoto, E. (2008). Inhibitory effects of opioids on compound action potentials in frog sciatic nerves and their chemical structures. Life sciences, 83(5), 198-207.
• Randall, D., Burggren, W., & French, K. (2001). Action Potentials. In Eckert Animal Physiology: Mechanisms and Adaptations (5th ed., Vol. 1, pp. 145-147). New York, New York: W.H. Freeman.
• Curran, A., & Department, B. (2014). Compound Action Potential. In Animal Physiology Lab - Biology 3810 (5th ed., Vol. 1, pp. 77-78). Denton, Texas: Eagle Images.
• Compound Action Potential of the Frog Sciatic Nerve. (2014, January 1). Retrieved March 7, 2015, from
BIOL 3810-502
LAB REPORT #1
COMPOUND ACTION POTENTIAL (5)
DATE PERFORMED: February 13th, 2015
LAB PARTNERS: #1-3
TA: JAMES CUMMINS
Introduction The sciatic nerve in the double pithed bullfrog is made up of a large bundle of various nerve fibers. Nerve fibers are defined by Eckert as “elongated cylindrical processes of a neuron along which action potentials are conducted” (G15, Eckert, 2001) and are vital to the successful conduction of this experiment. By stimulating the frog’s sciatic nerve with recording/stimulating electrodes in the nerve bath, students were able to observe the resultant electrical activity that occurred (CAP) through the PowerLab software. An understanding of neural anatomy is vital to maintain …show more content…
The absolute refractory period occurs either during or after the action potential when an electrical stimulus is completely unable to produce an excitation response in the neuron (nerve cell) no matter the strength of that second stimulus. The refractory period is the period of time following excitation that is necessary for a cell membrane to recover and be ready for a second electrical stimulus after it has returned to its original resting state (Curran, 2014). Relative refractory period is the period that according to the McGill Lab “immediately follows the absolute refractory period” when the action potential is unable to be initiated unless it is exposed to a stronger than average electrical stimulus (McGill, 2014). The refractory periods experiment involved the stimulation of the sciatic nerve at various intervals that progressively decreased by .5 ms each time before a new run was recorded. The end of the refractory period was marked by making a notation at which stimulus interval indicated a decrease in the magnitude of the second …show more content…
K., Ransom, B. R., & Waxman, S. G. (1991). Compound action potential of nerve recorded by suction electrode: a theoretical and experimental analysis. Brain research, 546(1), 18-32.
• DEPARTMENT, B. (2014, February 4). COMPOUND ACTION POTENTIAL: NERVE CONDUCTION. Retrieved March 7, 2015, from http://www.biopac.com/curriculum/pdf/a03.pdf
• Dalkilic, N., & Pehlivan, F. (1994). Derivatives and integrals of compound action potential of isolated frog sciatic nerves. J Ankara Med School, 16, 1147-1155.
• Mizuta, K., Fujita, T., Nakatsuka, T., & Kumamoto, E. (2008). Inhibitory effects of opioids on compound action potentials in frog sciatic nerves and their chemical structures. Life sciences, 83(5), 198-207.
• Randall, D., Burggren, W., & French, K. (2001). Action Potentials. In Eckert Animal Physiology: Mechanisms and Adaptations (5th ed., Vol. 1, pp. 145-147). New York, New York: W.H. Freeman.
• Curran, A., & Department, B. (2014). Compound Action Potential. In Animal Physiology Lab - Biology 3810 (5th ed., Vol. 1, pp. 77-78). Denton, Texas: Eagle Images.
• Compound Action Potential of the Frog Sciatic Nerve. (2014, January 1). Retrieved March 7, 2015, from