Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…
People are raving about the effectiveness of Yacon Syrup, especially those women going through premenopausal systems. The upward trend in recent years has been to live healthier and longer. One way to live longer is to lose weight. It would seem that more of us are overweight and obese people than not. Researchers have been steadily developing more and more weight loss supplements and regimes as a consequence of the change.…
Natalie Buttaccio Biol 251 Oliphant January 20, 2017 1. According to Bonnie Bassler in the video TedTalk, the human body is considered 90% prokaryotic because there are ten times more bacterial cells than human cells on a human being. Humans have approximately 30,000 genes, but you actually have ten times more bacterial genes. Bassler considers humans about 90% bacterial. These bacterial cells literally almost do everything, from digesting our food, making our vitamins, keeping us healthy, etc.…
The Effect of Change in Temperature Conditions on Enzymes Bacillus licheniformis Amylase and Aspergillus oryzae Amylase Over Time ____________________________________ Roxanna Flores PID: 5199946 Lab Section U26 Group Members: Roudabyna Taleb Abstract This laboratory was performed in order to be able to determine the effect of temperature over time on bacterial amylase Bacillus licheniformis and fungal amylase Aspergillus oryzae. Bacterial amylase was introduced into four singular test tubes at different temperatures. A starch solution was added to each to be able to observe the behavior of the enzyme.…
“It Takes Guts” is a documentary that talks about the obesity epidemic, and how microbes play a crucial role in affecting the overall size of an individual. “Microbes are defined as single-cell organisms so tiny that millions can fit into the eye of a needle. They are the oldest form of life on earth”. Microbes are mostly found in the guts. In fact, according to the documentary, geneticist Tim Spector states that we are creatures that evolved from microbes and we can not possibly live without them.…
The goal of this experiment was to determine the Michaelis constant (Km) and also the maximal velocity (Vmax) and the inhibition of alkaline phosphate. In order to accomplish these goals, 5 samples were used. Each sample contained different volumes of 0.2 m MPNPP (p-nitrophenylphosphate) and 0.2 M Tris-Hcl at a pH of 8.0. To each sample 0.2 mL of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance at a wavelength of 410 nm was recorded at time frame of 2 minutes for each solution.…
1. Glycolysis a. Indicate at what stage the compound is officially no longer a sugar, and why? The compound is no longer a sugar is at stage five.…
Balances the diet Passing gas can even help you to plan your diet better because our bodies react differently to different types of food. This is the reason why gases smell worse if you eat a lot of red mean. On the other side, the odor is more neutral if you diet consists of a lot of carbohydrates. Reduced pain in the abdomen…
The experiment calls for the extraction and purification of a protein. The protein chosen for the experiment was an amylase, specifically beta-amylase. Amylase protein can be typically found in plants and animals. The role of this protein is to hydrolyze organic materials, such as starch and dextrin, in order to form glucose, maltose, and limit dextrin. One distinction between beta amylase and alpha amylase is that beta amylase is not found in animal.…
Reflecting back to the experiment the purpose was to assess the amount of B-galactosidase enzyme present in Protein samples 1,2,3,4 and 5 that were taken from 5 different adult mouse tissue representatively; liver, heart, kidney, spleen and lung. GLB1, beta-galactosidase is a lysosomal enzyme that hydrolysis the terminal of beta D-galactose from substrates like lactose [1].Two methods were used to detect GLB1; SDS-PAGE and Western blotting. SDS-PAGE was used to separate proteins according to their electrophoretic mobility and their size. On the other hand Western blotting allowed us to investigate the molecular weight of the protein and the amount of protein present in the tissue sample. Figure 1 shows SDS- polyacrylamide gel stained with…
Disease Overview Type 1 or classic galactosemia is autosomal recessive disease that has the potential to become lethal. This disease causes the inability for newborns to process galactose within their liver. This can be fatal as rising levels of galactose within the body can be very toxic. Even individuals who survive the high level of galactose would obtain neurological damage. This disease has several variations, however, this particular manifestation is the result of gene that encode for galatctose 1-phosphate.…
Enzymes are proteins serving as catalysts that carry out thousands of chemical reactions that occur in living cells. A catalyst speeds up a chemical reaction by changing the rate of the reaction without being consumed by the reaction. In an enzyme - catalyzed reaction the substance acted upon that is called substrate binds to the active site of the enzyme. An enzyme-substrate complex is formed held by hydrophobic interactions, hydrogen bonds and ionic bonds. The substrate is converted to reaction products by catalytic action of the enzyme.…
The generalized process of digesting a large meal consists of consumption of food, progression through the digestive tract, absorption of nutrients, and secretion of waste. The process seems far from complex; however, at the cellular level digestion transpires in much more intricate detail. The human body must be supported by organic compounds that provide chemical balance. The steps and regulations of cellular digestion allow for generation of high energy products. Carbohydrates are the essential molecules needed to provide energy by facilitation of chemical reactions that release fuels for the body.…
Ryan Lobrovich 951194095 11/25/2015 Exploring the Basis of Non-Celiac Gluten Sensitivity Does gluten sensitivity exist, and if so how widely does it affect the general population? Having a gluten allergy can be a very real problem for the small percentage of people in the United States with celiac disease. Recently people without celiac disease have been attempting to find a connection between their physical well being regarding their gastrointestinal issues and a gluten-free diet. A large population have begun self diagnosing themselves with a new disorder called non-celiac gluten sensitivity (NCGS) to describe their bodily issues seemingly caused by gluten intake. Individuals with NCGS self-diagnose by removing food with gluten from their…
Assessing the body condition score (BCS) of a horse is needed in order to evaluate the physical health of the horse. The ideal BCS of many horses is between a 5 and 6, while a thoroughbred in the racing industry has an ideal BCS of a 4. While the BCS of a horse is a good way to determine the overall body condition of a horse, it does not tell whether or not the horse is getting the required nutrients in the appropriate amounts. The required nutrients can be calculated into the horse’s diet based on age, weight, and activity level. When there are mineral deficiencies in a horse, certain signs and symptoms may arise.…