Introduction
Enzymes are proteins that are biological catalysts that increases the rate of chemical reactions (JRank Articles). With the use of enzymes it can accelerate certain reaction rates at a faster past then if the reaction was moving at it natural speed (JRank Articles). The activity of enzymes are control by many things like environment, inhibitors, and regulatory binding sites that happens on the enzyme(JRank Articles).The overall reaction in the natural setting which was the basic reaction in this experiment was in a time period of twenty minutes the solution went from a pale peach to a darker more copper color. The hypothesis/prediction for the basic reaction, control problem one, and control problem two is, if benzoquinone is present …show more content…
For the first experiment, we had three test tube set up one was a basic reaction and the others were two control groups. The basic test tube had 1.4mL of a buffer, 0.5mL substrate, and 0.1 mL enzymes, one control test tube had only 1.9mL buffer and 0.1 mL enzyme, while the other control test tube had 1.5mL buffer and 0.5 mL. Each of the three test tubes had sat in the water bath for twenty minutes while checking on them for every five minutes to be able to observe any color changes. For the temperature experiment, we did two test tube one hot and one for cold, both had 14mL of a buffer, 0.5 mL of the substrate, and 0.1 mL of the enzyme. Each was sat for twenty minutes checking every five minutes for a color change. The only different in this experiment is that one test tube sat in an ice bucket and the other test tube sat in a beaker on a hot plate. For the pH optimum experiment, my partner Gabby and I choose to do two test tube of one having 1.4mL of the very basic, Boric acid, and the other having 1.4mL of the very acid, 5 NHCl. Both test tubes had 0.5 mL of the substrate and 0.1 mL of the enzyme. They both sat for twenty minutes in the water bath and checking every five
Enzymes are proteins that are biological catalysts that increases the rate of chemical reactions (JRank Articles). With the use of enzymes it can accelerate certain reaction rates at a faster past then if the reaction was moving at it natural speed (JRank Articles). The activity of enzymes are control by many things like environment, inhibitors, and regulatory binding sites that happens on the enzyme(JRank Articles).The overall reaction in the natural setting which was the basic reaction in this experiment was in a time period of twenty minutes the solution went from a pale peach to a darker more copper color. The hypothesis/prediction for the basic reaction, control problem one, and control problem two is, if benzoquinone is present …show more content…
For the first experiment, we had three test tube set up one was a basic reaction and the others were two control groups. The basic test tube had 1.4mL of a buffer, 0.5mL substrate, and 0.1 mL enzymes, one control test tube had only 1.9mL buffer and 0.1 mL enzyme, while the other control test tube had 1.5mL buffer and 0.5 mL. Each of the three test tubes had sat in the water bath for twenty minutes while checking on them for every five minutes to be able to observe any color changes. For the temperature experiment, we did two test tube one hot and one for cold, both had 14mL of a buffer, 0.5 mL of the substrate, and 0.1 mL of the enzyme. Each was sat for twenty minutes checking every five minutes for a color change. The only different in this experiment is that one test tube sat in an ice bucket and the other test tube sat in a beaker on a hot plate. For the pH optimum experiment, my partner Gabby and I choose to do two test tube of one having 1.4mL of the very basic, Boric acid, and the other having 1.4mL of the very acid, 5 NHCl. Both test tubes had 0.5 mL of the substrate and 0.1 mL of the enzyme. They both sat for twenty minutes in the water bath and checking every five