Exploring Bacterial Transformation
Ian Nall
BIO 181 | Section #73116 | Wednesday 1:30-4:15pm
Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either ampicillin or not as well as with a ribosome known as arabinose. The E. coli without pGLO died in the ampicillin and the ones with pGLO survived after a period of incubation, with the one that had arabinose glowing under ultraviolet light. This was the expected result due to GFP adding a resistance to ampicillin in the plasmid.
Introductions
Escherichia coli …show more content…
coli? The idea of this particular experiment is to modify E. coli in such a way that it takes on the gene of another organism. This organism is a jellyfish which has a protein that causes it to become fluorescent in the right conditions (Hayden-McNeil, 2015). So what the experiment intends to do is to take an easily transferrable form of this protein, in the form of a plasmid, and get it to go into a group of E. coli and see what happens. The hypothesis is if it works, the protein will be present and if under the right condition, i.e. it is under ultraviolet(UV) light and has arabinose a ribosome that activates the gene the E. coli will then become fluorescent themselves. On top of that the plasmid also allows E. coli to become resistant to ampicillin, meaning that a good portion will survive when present to an otherwise deadly substance. The null hypothesis then would be that in the presence of the above factors nothing will happen, then in the presence of ampicillin the E. coli cannot survive and they will die during the incubation
Ian Nall
BIO 181 | Section #73116 | Wednesday 1:30-4:15pm
Abstract This experiment was designed to test the properties of bacterial transformation. The bacteria used was E. coli, the protein being added to it is called Green Fluorescent Protein or GFP from a jellyfish the Aequorea victoria. The GFP, in the form of a plasmid called pGLO, was added to a sample of E. coli and a control was created of E. coli without it. The E. coli was then placed in either ampicillin or not as well as with a ribosome known as arabinose. The E. coli without pGLO died in the ampicillin and the ones with pGLO survived after a period of incubation, with the one that had arabinose glowing under ultraviolet light. This was the expected result due to GFP adding a resistance to ampicillin in the plasmid.
Introductions
Escherichia coli …show more content…
coli? The idea of this particular experiment is to modify E. coli in such a way that it takes on the gene of another organism. This organism is a jellyfish which has a protein that causes it to become fluorescent in the right conditions (Hayden-McNeil, 2015). So what the experiment intends to do is to take an easily transferrable form of this protein, in the form of a plasmid, and get it to go into a group of E. coli and see what happens. The hypothesis is if it works, the protein will be present and if under the right condition, i.e. it is under ultraviolet(UV) light and has arabinose a ribosome that activates the gene the E. coli will then become fluorescent themselves. On top of that the plasmid also allows E. coli to become resistant to ampicillin, meaning that a good portion will survive when present to an otherwise deadly substance. The null hypothesis then would be that in the presence of the above factors nothing will happen, then in the presence of ampicillin the E. coli cannot survive and they will die during the incubation