Essay Bacterial Gene Expression And Its Effects On The Environment

2370 Words Apr 28th, 2016 10 Pages
Abstract Many number of medicines, like growth hormones and insulin, are a possibility because of techniques like transformation of genetically engineered bacteria. Although, manipulating bacterial gene expression has many great implications, it can also be quite harmful. By manipulating gene expression of bacteria, such as Escherichia coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was conducted in order to transform E.coli into an antibiotic-resistant bacteria by introducing a plasmid, lux and pUC18, containing a gene for the resistance to Ampicillin. The procedure had four basic steps: treating bacterial cells with CaCl2 solution in order for uptake of plasmid DNA to be enhanced; incubation of CaCl2 -treated cells with plasmid DNA; selections of cells that took up plasmid DNA; and examination of cultures. The findings showed that the lux and pUC18 treatments, which had been exposed to Ampicillin, had Colonial growth, while the treatments which had not been exposed to Ampicillin had Lawn growth. On the other hand, the treatments that had been exposed to Ampicillin but did not contain a plasmid, had no growth. Findings showed that transformation was successful with the introduction of both plasmid DNA. Potential experimental errors on transformation are discussed.

Introduction Every day there are advances in Biotechnology and such advances are allowing…

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