The potential for the inhibition of anti-apoptotic and RNAi pathways to enhance in vitro baculovirus biopesticide production
Summary
Baculoviruses are target specific virus infected a number of plant pests especially the lepidopterous pests. Baculovirus biopesticides have been used worldwide for decades. Although many have been done to enhance yields of baculovirus in vitro production, their large-scale production poses certain difficulties that limited the use. Apart from optimization of cell lines, culture medium, bioreactors and culture mode, the key aspect is to improve yields of occlusion bodies (OB) of baculovirus in which host cell modifications may provide a biological approach for furthering enhancing the yields. Ideally, improving …show more content…
The NPVs are phylogenetically subdivided into two groups, type I and type II. The main difference between them is that type I NPVs contain the fusion protein GP64, needed for virus entry into the cell and for cell-to-cell transmission, while type II NPVs lack GP64 and instead contain a generic fusion protein, called F protein, with the same role as GP64. This protein can also be found in some vertebrate viruses (Szewczyk et al., …show more content…
Suitable bioreactor types for suspension-adapted animal cells include the stirred-tank reactor (STR), airlift reactor (ALR), and wave/rocking-motion reactors, in which STR is the most widely used (Contreras‐Gómez et al., 2014). There are over 20 different kinds of disposable bioreactor in production of biotechnology products in which considerably large STRs up to 2000 L are also commonly used (Eibl et al., 2010). Insect cell cultures have been reported at scales of at least 600 L in STRs and 500 L in rocking-motion plastic bag-based single-use bioreactors (SUBs) (Contreras‐Gómez et al., 2014). Apart from the bioreactors used, the culture mode is also an important consideration when optimizing a process. There are usually three types of culture mode, batch, fed-batch and perfusion (Fig. 2). Fed-batch culture is an improved version of a basic batch culture involving one or more nutrient supplements added either pulse-wise or continuously as a culture reaches higher cell densities with a corresponding reduction in nutrient levels. Fed-batch is often suggested to be best option for mass production due to its simplicity and relatively good yield compared to batch or the more complicated perfusion processes (Elias et al.,
Summary
Baculoviruses are target specific virus infected a number of plant pests especially the lepidopterous pests. Baculovirus biopesticides have been used worldwide for decades. Although many have been done to enhance yields of baculovirus in vitro production, their large-scale production poses certain difficulties that limited the use. Apart from optimization of cell lines, culture medium, bioreactors and culture mode, the key aspect is to improve yields of occlusion bodies (OB) of baculovirus in which host cell modifications may provide a biological approach for furthering enhancing the yields. Ideally, improving …show more content…
The NPVs are phylogenetically subdivided into two groups, type I and type II. The main difference between them is that type I NPVs contain the fusion protein GP64, needed for virus entry into the cell and for cell-to-cell transmission, while type II NPVs lack GP64 and instead contain a generic fusion protein, called F protein, with the same role as GP64. This protein can also be found in some vertebrate viruses (Szewczyk et al., …show more content…
Suitable bioreactor types for suspension-adapted animal cells include the stirred-tank reactor (STR), airlift reactor (ALR), and wave/rocking-motion reactors, in which STR is the most widely used (Contreras‐Gómez et al., 2014). There are over 20 different kinds of disposable bioreactor in production of biotechnology products in which considerably large STRs up to 2000 L are also commonly used (Eibl et al., 2010). Insect cell cultures have been reported at scales of at least 600 L in STRs and 500 L in rocking-motion plastic bag-based single-use bioreactors (SUBs) (Contreras‐Gómez et al., 2014). Apart from the bioreactors used, the culture mode is also an important consideration when optimizing a process. There are usually three types of culture mode, batch, fed-batch and perfusion (Fig. 2). Fed-batch culture is an improved version of a basic batch culture involving one or more nutrient supplements added either pulse-wise or continuously as a culture reaches higher cell densities with a corresponding reduction in nutrient levels. Fed-batch is often suggested to be best option for mass production due to its simplicity and relatively good yield compared to batch or the more complicated perfusion processes (Elias et al.,