Reflecting back to the experiment the purpose was to assess the amount of B-galactosidase enzyme present in Protein samples 1,2,3,4 and 5 that were taken from 5 different adult mouse tissue representatively; liver, heart, kidney, spleen and lung. GLB1, beta-galactosidase is a lysosomal enzyme that hydrolysis the terminal of beta D-galactose from substrates like lactose [1].Two methods were used to detect GLB1; SDS-PAGE and Western blotting. SDS-PAGE was used to separate proteins according to their electrophoretic mobility and their size. On the other hand Western blotting allowed us to investigate the molecular weight of the protein and the amount of protein present in the tissue sample.
Figure 1 shows SDS- polyacrylamide gel stained with
Figure 1 shows SDS- polyacrylamide gel stained with