Introduction
The aim of the experiment was to investigate whether different formulations of aspirin would affect its bioavailability. This required the use of normal aspirin tablets, aspirin tablets with sodium bicarbonate, soluble aspirin, and enteric coated aspirin. Depending on which group the students were allocated they administered different formulations of aspirin. The pH and the concentration of salicylate in each urine sample, collected at different time points, was determined after administering the drug. A standard curve for salicylate was generated to calculate its concentration in each urine sample by comparing the optical density using the following equation: change in optical density (from control)×1/(gradient of graph)=concentration …show more content…
A high salicylate concentration indicates that more acetylsalicylic acid, aspirin, is absorbed. This is because the metabolism of acetylsalicylic acid leads to the production of salicylic acid and salicyluric acid by hydrolysis and conjugation of glycine respectively. Hence, in a urine sample the detected concentration of salicylate reflects the amount of salicylic acid and salicyluric acid excreted, which provides a method for measuring the amount of acetylsalicylic acid …show more content…
This could be due to confounding variables or experimental error as explained above. Nonetheless, it is statistically significant as none of the error bars on the graph overlap. The aspirin tablet group also had a similar pattern and the results were in parallel to the soluble aspirin group. However, the group that administered enteric coated aspirin had the lowest amount of salicylate excreted and there was a continuous decrease in subsequent samples. The first sample contained 1.725mg of salicylate, whereas the third sample contained
The aim of the experiment was to investigate whether different formulations of aspirin would affect its bioavailability. This required the use of normal aspirin tablets, aspirin tablets with sodium bicarbonate, soluble aspirin, and enteric coated aspirin. Depending on which group the students were allocated they administered different formulations of aspirin. The pH and the concentration of salicylate in each urine sample, collected at different time points, was determined after administering the drug. A standard curve for salicylate was generated to calculate its concentration in each urine sample by comparing the optical density using the following equation: change in optical density (from control)×1/(gradient of graph)=concentration …show more content…
A high salicylate concentration indicates that more acetylsalicylic acid, aspirin, is absorbed. This is because the metabolism of acetylsalicylic acid leads to the production of salicylic acid and salicyluric acid by hydrolysis and conjugation of glycine respectively. Hence, in a urine sample the detected concentration of salicylate reflects the amount of salicylic acid and salicyluric acid excreted, which provides a method for measuring the amount of acetylsalicylic acid …show more content…
This could be due to confounding variables or experimental error as explained above. Nonetheless, it is statistically significant as none of the error bars on the graph overlap. The aspirin tablet group also had a similar pattern and the results were in parallel to the soluble aspirin group. However, the group that administered enteric coated aspirin had the lowest amount of salicylate excreted and there was a continuous decrease in subsequent samples. The first sample contained 1.725mg of salicylate, whereas the third sample contained