Aseptic technique:
1. Aseptic technique is a method carried out by medical laboratory scientists. This procedure is used to kill or inhibit pathogens, and is fulfilled by using antiseptics. Antiseptics are chemical agents which prevent or eliminate microbial infections; however the chemical agents do not damage the host tissue.
2. One of the best ways to achieve aseptic method remains maintaining a hygienic environment, but also a personal one. Wearing gloves and laboratory coat is also a good practice to prevent the contamination. The Autoclave is technical equipment which is used for the sterilization of tools used in the laboratory. The sterilization is achieved by the use of pressurized steam. The surface of working must be kept clean …show more content…
Escherichia coli is one of the bacteria which is naturally found within animal guts, water, soil, and aids digestion. But there are certain strains of bacteria that are harmful to the body and cause bacterial infections. They are often found in some vegetables or meats.
2. When E. coli leaves the natural habitat, and penetrate other organs is causing infection. E. coli causes infections in the digestive system or in urinary tract. Infections that some strains of E. coli might cause can have as an effect the following symptoms: stomach cramps, nausea and anddiarrhea, while some infections can be asymptotic. Very rarely can cause kidney failure or even severe haematological problems.
3. Most strains of this bacteria are harmless, but there are several strains of E. coli which do cause diseases in humans (e.g. E. coli O1257: H7 ). By comparing a pathogenic genome with a non-one, the virulence genes can be identified.
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5. Escherichia coli is found in the human colon, whose normal temperature is around 37 degrees celsius ((98.6 degrees Fahrenheit) .The optimal temperature for E. coli to grow is 37 C, because grows best at average temperature of its host. This is a mesophilicbacteria, which can develop at temperatures between 10 and 45 degrees Celsius. But this development will not be as fast as that obtained at 37 degrees celsius. This is the reason why during the practical, the plates inoculated with presumed E. coli were incubated at 37 C, to facilitate …show more content…
A selective growth media is composed by specific ingredients to inhibit the growth of positive-gram bacteria, by killing non-desired organisms. MacConkey agar contains bile salts and crystal violet, which impede the growth of positive-gram microorganisms, while allow the growth of gram negative bacteria. A differential growth media, as the name suggests, distinguish between the types of microorganisms that grow on the same medium. This distinction is made possible by the biochemical characteristics of the organism in the presence of certain indicators or nutrients (such as neutral red, phenol red, eosin y, or methylene blue) which “identify lactose fermenters as red colonies and non-fermenters as white or
1. Aseptic technique is a method carried out by medical laboratory scientists. This procedure is used to kill or inhibit pathogens, and is fulfilled by using antiseptics. Antiseptics are chemical agents which prevent or eliminate microbial infections; however the chemical agents do not damage the host tissue.
2. One of the best ways to achieve aseptic method remains maintaining a hygienic environment, but also a personal one. Wearing gloves and laboratory coat is also a good practice to prevent the contamination. The Autoclave is technical equipment which is used for the sterilization of tools used in the laboratory. The sterilization is achieved by the use of pressurized steam. The surface of working must be kept clean …show more content…
Escherichia coli is one of the bacteria which is naturally found within animal guts, water, soil, and aids digestion. But there are certain strains of bacteria that are harmful to the body and cause bacterial infections. They are often found in some vegetables or meats.
2. When E. coli leaves the natural habitat, and penetrate other organs is causing infection. E. coli causes infections in the digestive system or in urinary tract. Infections that some strains of E. coli might cause can have as an effect the following symptoms: stomach cramps, nausea and anddiarrhea, while some infections can be asymptotic. Very rarely can cause kidney failure or even severe haematological problems.
3. Most strains of this bacteria are harmless, but there are several strains of E. coli which do cause diseases in humans (e.g. E. coli O1257: H7 ). By comparing a pathogenic genome with a non-one, the virulence genes can be identified.
4. ???????
5. Escherichia coli is found in the human colon, whose normal temperature is around 37 degrees celsius ((98.6 degrees Fahrenheit) .The optimal temperature for E. coli to grow is 37 C, because grows best at average temperature of its host. This is a mesophilicbacteria, which can develop at temperatures between 10 and 45 degrees Celsius. But this development will not be as fast as that obtained at 37 degrees celsius. This is the reason why during the practical, the plates inoculated with presumed E. coli were incubated at 37 C, to facilitate …show more content…
A selective growth media is composed by specific ingredients to inhibit the growth of positive-gram bacteria, by killing non-desired organisms. MacConkey agar contains bile salts and crystal violet, which impede the growth of positive-gram microorganisms, while allow the growth of gram negative bacteria. A differential growth media, as the name suggests, distinguish between the types of microorganisms that grow on the same medium. This distinction is made possible by the biochemical characteristics of the organism in the presence of certain indicators or nutrients (such as neutral red, phenol red, eosin y, or methylene blue) which “identify lactose fermenters as red colonies and non-fermenters as white or