Artificial Insemination Case Study

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Artificial insemination (AI), is a procedure often used in livestock production to facilitate and maximize breeding rates. The first official AI was performed on a bitch in 1784. by an Italian physiologist by the name of Lazzaro Spallanzaniand resulted in the birth of 3 puppies (Ombelet & Van Robays 2015). According to Donoghue & Wishart (2000), in commercial avian species, AI has been a significant reproduction since the 1960’s, namely in commercial turkeys due to increasing size of males, however development on AI began in the 1930’s. In chickens, semen is collected from the rooster and can be mixed with an extender, to be used immediately or stored for future use. Poultry semen, unlike semen of domestic mammalian, is prone to freeze damage, therefore, rather than being cryogenized, rooster semen is
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However, in a study comparing frozen individual and pooled roster semen, viability was slightly greater in single sourced samples (Herrara et al. 2005). Although this experiment didn’t use frozen storage, it could be expected that the results would have been similar in refrigerated semen.
It was expected that the fresh individual semen would be most successful, in both aspects studied, followed by the fresh pooled semen, the stored individual semen, and finally, the stored pooled sample. The reason for this pattern was due to a theory that refrigerating semen would cause it too lose potency, while spermatozoa from different roosters would compete and attack each other, therefore, following this presumption, fresh semen would be more successful than its stored counterpart, while individual semen would be more successful than pooled.
These predictions were almost accurate, however, it was treatment 3, the individual stored semen, which proved to be the least successful in both percent fertility and total amount of fertile eggs produced over the collection

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