Abstract
Objectives: The anticonvulsant effect of cannabinoid compound illustrated in various models of seizure and epilepsy. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. Also, there is no data regarding the effect of co-administration of cannabinoid type 1 (CB1) receptor agonists and BK channels antagonists in the acute models of seizure in mice. In this study, the effects of arachidonyl-2′-chloroethylamide (ACEA), as a CB1 receptor agonist, and BK channel antagonist, paxilline, either alone or in combination were investigated.
Materials and Methods: The pentylenetetrazole (PTZ) or maximal electroshock (MES) acute models of …show more content…
Comparison of various time courses by Kruskal–Wallis test revealed a significant difference in PTZ-induced seizure threshold (P<0.001) when the test was performed at different times after drug injections. Further analysis revealed that ACEA at the dose of 5 and 10 mg/kg significantly increased seizure threshold when it was administered 30 min before PTZ test (P<0.01compared to control …show more content…
Effect of ACEA and paxilline in the electroshock-induced seizure model
The control group which received vehicle exhibit 10% protection against electroshock-induced seizure .Effect of ACEA and paxilline in the electroshock-induced seizure model.The results have been shown in Table 2. Administration of 0.5, 1 ,5,10 and 50 mg/kg of ACEA resulted in 10, 10, 30 ,40and 85% protection against electroshock, respectively. Administration of paxilline at doses of 0.5, 1, 5, 10 and 50 mg/kg produced 10, 30, 40, 25 and 50% protection, respectively.
Effect of ACEA alone and in combination with Paxilline in electroshock-induced seizure model
The data shown in Table (2) describes the percent protection against shock-induced seizure in mice treated with different doses of ACEA alone and in combination with various doses of paxilline. Administration of 1, 5, 10 and 50 mg/kg of ACEA resulted in 10, 30, 40 and 85% protection against electroshock, respectively. Co-administration of ACEA and paxilline showed significant interaction in anticonvulsant activity (P = 0.032; Table 2).
Objectives: The anticonvulsant effect of cannabinoid compound illustrated in various models of seizure and epilepsy. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. Also, there is no data regarding the effect of co-administration of cannabinoid type 1 (CB1) receptor agonists and BK channels antagonists in the acute models of seizure in mice. In this study, the effects of arachidonyl-2′-chloroethylamide (ACEA), as a CB1 receptor agonist, and BK channel antagonist, paxilline, either alone or in combination were investigated.
Materials and Methods: The pentylenetetrazole (PTZ) or maximal electroshock (MES) acute models of …show more content…
Comparison of various time courses by Kruskal–Wallis test revealed a significant difference in PTZ-induced seizure threshold (P<0.001) when the test was performed at different times after drug injections. Further analysis revealed that ACEA at the dose of 5 and 10 mg/kg significantly increased seizure threshold when it was administered 30 min before PTZ test (P<0.01compared to control …show more content…
Effect of ACEA and paxilline in the electroshock-induced seizure model
The control group which received vehicle exhibit 10% protection against electroshock-induced seizure .Effect of ACEA and paxilline in the electroshock-induced seizure model.The results have been shown in Table 2. Administration of 0.5, 1 ,5,10 and 50 mg/kg of ACEA resulted in 10, 10, 30 ,40and 85% protection against electroshock, respectively. Administration of paxilline at doses of 0.5, 1, 5, 10 and 50 mg/kg produced 10, 30, 40, 25 and 50% protection, respectively.
Effect of ACEA alone and in combination with Paxilline in electroshock-induced seizure model
The data shown in Table (2) describes the percent protection against shock-induced seizure in mice treated with different doses of ACEA alone and in combination with various doses of paxilline. Administration of 1, 5, 10 and 50 mg/kg of ACEA resulted in 10, 30, 40 and 85% protection against electroshock, respectively. Co-administration of ACEA and paxilline showed significant interaction in anticonvulsant activity (P = 0.032; Table 2).