Introduction
The topic of the lab was to introduce the basics of enzymes. The topic is important because they are in every living cell and because of enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the temperature, then the enzyme reaction time will also increase. For part 2 section B my hypothesis was if we change the level of the pH, then the ability of the enzyme will be changed. In part 2 section C my hypothesis was if you increase the concentration, …show more content…
First for this experiment start by getting one Lactaid pill and crush it into powder with a mortar and pestle. Then add powder to 5 mL of phosphate buffer and vortex to dissolve the pill. Put 1 mL of the solution into 5 of the microcentrifuge tubes and 1 mL of water in a microcentrifuge tube and centrifuged these tubes for 5 minutes. Then combine the solutions into one clean test tube and put it ice. Next combine 3.5 mL of buffer and 0.5 of your substrate. Then obtain 0.5 sodium carbonate, which was the stop solution. Next add 0.5 mL of enzyme to the buffer and substrate and start the timer. Wait until a pale yellow color appears to add the stop solution and also stop the timer. Then determine the absorbance by using the Spec 20 and recorded the data. The control for this experiment was the amount of enzyme solution that was …show more content…
When given an unknown solution you can use Excel and determine the concentration or absorbance and determine what the substance is. The results in part two shows that temperature, pH,and concentration can affect enzyme activity. This means the environment has a key role in if the enzyme activity will decrease or increase. Every enzyme assay we did was wrong due to the fact we did not ice our solution long enough. Improvements for this would be to come around to every group and make sure the students are doing
The topic of the lab was to introduce the basics of enzymes. The topic is important because they are in every living cell and because of enzymes chemistry is allowed in the cell. They do this by providing the energy needed for chemical bonds to break down and lets molecules interact with each other. For part 1 of the lab my hypothesis was if you increase the concentration, then the absorbance will also increase. For part 2 section A my hypothesis was if you increase the temperature, then the enzyme reaction time will also increase. For part 2 section B my hypothesis was if we change the level of the pH, then the ability of the enzyme will be changed. In part 2 section C my hypothesis was if you increase the concentration, …show more content…
First for this experiment start by getting one Lactaid pill and crush it into powder with a mortar and pestle. Then add powder to 5 mL of phosphate buffer and vortex to dissolve the pill. Put 1 mL of the solution into 5 of the microcentrifuge tubes and 1 mL of water in a microcentrifuge tube and centrifuged these tubes for 5 minutes. Then combine the solutions into one clean test tube and put it ice. Next combine 3.5 mL of buffer and 0.5 of your substrate. Then obtain 0.5 sodium carbonate, which was the stop solution. Next add 0.5 mL of enzyme to the buffer and substrate and start the timer. Wait until a pale yellow color appears to add the stop solution and also stop the timer. Then determine the absorbance by using the Spec 20 and recorded the data. The control for this experiment was the amount of enzyme solution that was …show more content…
When given an unknown solution you can use Excel and determine the concentration or absorbance and determine what the substance is. The results in part two shows that temperature, pH,and concentration can affect enzyme activity. This means the environment has a key role in if the enzyme activity will decrease or increase. Every enzyme assay we did was wrong due to the fact we did not ice our solution long enough. Improvements for this would be to come around to every group and make sure the students are doing