Apple Juice Enzyme Lab

Improved Essays
Introduction
Problem Statement: Which enzyme will produce the most juice from applesauce pectinase, cellulase, or the mixture of pectinase/cellulase?
Hypothesis: If Pectinase, Cellulase and combination of Pectinase and Cellulase are used for extracting juice from applesauce, than the Pectinase will produce more juice because it is already mainly used for extracting juice from fruit in the real world. Independent Variable: The enzymes (pectinase, cellulase, and the combination of pectinase/cellulase)
Dependent Variable: The amount of juice produced from the juice production
Control Group: Water instead of enzymes
Factor(s) Held Constant: The amount of applesauce in each cup, same cups, amount of enzyme put into the applesauce, time spent
…show more content…
But what are enzymes? Enzymes are proteins that speed up the rate of (chemical) reactions and also happen to be an important part of fruit juice making and wine. The food and wine-making industries use these enzymes with the enzyme amylase to degrade starch and to clarify solutions. There are two different enzymes being tested in this lab in order to see which enzyme produces more juice from applesauce; Pectinase and Cellulase. Cellulase is a class of enzymes produced by the fungi bacteria and protozoans that generate cellulolysis This process is actually the hydrolysis of cellulose The activity of the cellulase is to break down cellulose and convert it to into beta-glucose bacteria from herbivores make that type of cellulase. Cellulase is a carbohydrate that can be found in the cellular structure of a plant.
Pectinse, also known as pectin, is an enzyme that breaks down plant cell walls. Pectinase is also used when fruit is turned into pulp and then extracted to remove its juice. Also, the food industry use pectin to soften fruit rinds to make peeling them easier as well as fermenting coffee and tea. On a cellular level, this enzyme is used with other enzymes that degrade cell walls to reduce toxic chemicals in such processes like digestion or
…show more content…
The problem statement of the experiment was which enzyme will produce the most juice from applesauce pectinase, cellulase, or the mixture of pectinase/cellulase? The hypothesis stated, if Pectinase, Cellulase and combination of Pectinase and Cellulase are used for extracting juice from applesauce, than the Pectinase will produce more juice because it is already mainly used for extracting juice from fruit in the real world. However that was not the case when the combination of Pectinase and Cellulase was added to this experiment because it took the lead and had the most apple juice produced from the applesauce. The total number of milliliters produced from the combination of Pectinase and Cellulase was 58 mL while the total number of milliliters from Pectinase alone is 54 mL, a difference of 4 mL. These two totals created a large gap between the juice production from water and the juice production from

Related Documents

  • Great Essays

    Amylase Experiment

    • 1123 Words
    • 5 Pages

    The errors of the experiment are unknown and cannot be accurately stated. The possible improvements for the experiment could include that there are more intervals of temperature tested, pH levels could be tested, and the concentration of amylase put into the glucose…

    • 1123 Words
    • 5 Pages
    Great Essays
  • Improved Essays

    What predictions would you make about the enzyme activity if you were to continue to change each of the factors in Parts A – E? The predictions that would be concluded about the enzyme activity if it were to continue and change each of the factors in Parts A – E, would be that enzyme activity will decrease due to the optimal temperature, pH, concentrations, and metal salt concentration not being obtained. How does changing the concentration of enzyme affect the rate of decomposition of H2O2?…

    • 680 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Jello-O Enzyme Lab

    • 581 Words
    • 3 Pages

    Enzymes are protein molecules that significantly speed up the rate of virtually all of the chemical reactions that take place within the cell. In the following lab was to examine an enzyme that is found in pineapples. That enzyme is bromelain its breaks down protein into their protein into their amino acid by a process of hydrolysis. They are found in tropical areas like Hawaii, Thailand, and Brazil. For the first experiment the hypothesis was that the when gelatin was added to the water, fresh pineapple juice, and canned pineapple juice and submerged into the ice chest the solutions would turn into Jell-O.…

    • 581 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    Sweta Soni 2/22/15 Biochem Lab Experiment 5 Introduction The purpose of Experiment 5-1 is to separate a mixture of three molecules—blue dextran, cytochrome c, and potassium chromate—through the use of gel-filtration chromatography. The volume at which each molecule elutes will be recorded in order to determine the accuracy of separation. The purpose of Experiment 5-2 is to purify the E. coli enzyme, β-Galactosidase through the use of affinity chromatography on P-aminobenzyl-1-thio-β-D-galactopyranoside agarose, which is a good substrate for the enzyme.…

    • 363 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Jell-O Experiment

    • 331 Words
    • 2 Pages

    The purpose of this experiment was to identify what the effect type of fruit had on the consistency of Jell-O. The Jell-O with apple slices in it had the hardest consistency, and the pennies dropped into the Jell-O/apple mixture dropped an average of .07 inches into the container. The pennies in the mango and Jell-O mixture dropped in an average of 1.1875 inches in, and the pennies dropped an average of 1.4875 inches into the pineapple and Jell-O mixture, meaning the apples and Jell-O solution’s pennies sunk in the least. The penny was not able to break through the surface as much because the solution had a tougher consistency than the pineapple Jell-O solution and the mango Jell-O solution. All of the data shown in table 1 and graph 1 supported…

    • 331 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Catalase Experiment

    • 881 Words
    • 4 Pages

    The enzyme being used in this experiment is catalase. Catalase is an antioxidant enzyme that helps to…

    • 881 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Catalase Lab

    • 614 Words
    • 3 Pages

    Studying the reaction of the enzyme catalase in different temperatures The purpose of this experiment was to measure the speed and the effectiveness of the enzyme catalase. The enzyme catalase purpose is to break down harmful substances and turn them into oxygen and water. The substance in this environment was hydrogen peroxide, and the study was to see how well the chicken liver would work if it was in a boiled environment, room temperature environment, an acid immersed environment and a basic environment.…

    • 614 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Catalase Enzyme Lab Write-Up Our goal with this lab is to discover how enzymes affect the production and destruction of harmful chemicals our body makes into harmless chemicals. Our body contains hundreds of enzymes in our cells and this lab will show us how they interact with parts and materials of our bodies. The enzyme we will be focusing on is called catalase. It’s job for the body is to break down hydrogen peroxide a harmful substance into two harmless substances oxygen and water.…

    • 809 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    M & M Research Paper

    • 443 Words
    • 2 Pages

    M & M Three oranges were cut in half and then placed over the juicer. The oranges were then pressed down, while being twisted allowing the juices from the oranges to be collected in the tray below. The orange juice in the trays were then poured into a 250ml beaker with cheese cloth on top of it. The cheesecloth caught the larger pieces of pulp from the oranges while allowing the juice to pass. The orange juice was then transferred into six 1.5ml centrifuge tubes.…

    • 443 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Enzyme Lab Report

    • 537 Words
    • 3 Pages

    The objective of this experiment is to recognize how the introduction of an acid affects the activities of an enzyme. MATERIALS REQUIRED: Meat, acidic foods, potatoes, alkaline foods, a scaled beaker, yeast, boiling tube, hydrogen peroxide, 100 milliliter graduated cylinder, and a dropper HYPTHOSIS: If the pH level can affect the activity of an enzyme, then…

    • 537 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    Discussion: The specific purpose for this study was to see how enzyme activity would change as there was a change in temperature and pH. Both temperature and pH were hypothesized to have an affect on enzyme activity. Each had an optimal temperature. At this point, the rate of enzyme activity would stop increasing and begin to decrease. For temperature, it was hypothesized that the optimal temperature would be 48°C.…

    • 1717 Words
    • 7 Pages
    Superior Essays
  • Superior Essays

    Albumin Synthesis

    • 1183 Words
    • 5 Pages

    Through this experiment we measured how fast a chemical reaction occurs, by changing the degradation rate in Albumin when added to different enzymes. Albumin is a protein found in egg white, which is considered to have important storage and nutritional functions. Albumin has also been used in medicine to treat heavy metal intoxication. We ran Albumin through four different conditions to see which would make the Albumin degrade faster. We predicted that the Albumin would degrade with pepsin the fastest since it is essential for digestion of substances in the stomach.…

    • 1183 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of…

    • 753 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Introduction: Enzymes have a huge effect on the human body and other organisms. Enzymes are catalysts that are used to accelerate the reaction process by lowering the activation energy. For this experiment the catechol oxidase was extracted from potatoes. The enzyme is called catechol oxidase because the oxygen is reacting to catechol. Catechol oxidase causes the browning of the fruits and vegetables because the compound is toxic to the bacteria.…

    • 1142 Words
    • 5 Pages
    Improved Essays
  • Great Essays

    Sugar Dissolving Lab

    • 1835 Words
    • 8 Pages

    PURPOSE/QUESTION The question that we will answer using this laboratory report is, “How is the rate of dissolving sugar affected by changing the temperature of the water, changing the rate of stirring, and changing the size of the crystal?” The independent variable of each laboratory experiment is different, however they are all being changed to get consistent and effective results for one dependent variable. The three independent variables are the temperature of water, rate of stirring and size of the crystal and the dependent variable of the all the experiments is the rate of sugar dissolving. HYPOTHESES Below are the laboratory experiment hypotheses that we have formed as a group to figure out what will happen to the rate of dissolving under…

    • 1835 Words
    • 8 Pages
    Great Essays

Related Topics