Antisera evaluation: bacterial agglutination.
Introduction and Aim:
Agglutination is coalescing of antigen bearing cells, microorganisms or particles in the presence of specific antibodies, in a suspension. Antiserum is a serum containing antibodies which are specific for one or multiple antigens. Direct agglutination, a process by which antigens detect and bind directly to specific antibodies in serum against a microbe. Hence, the aim of this experiment is to qualitatively and semi-quantitatively analyse antisera by using direct bacterial agglutination techniques. The experiment will further determine the titre of antisera and the specificity of antibodies produced against two antigens (bacteria): Brucella abortus (B. abortus) and Brucella …show more content…
Involving the vaccination of proven naïve rabbits with an antigen enabling an immune response brought about by the rabbit identifying this anti-gen as foreign. Blood collection at various intervals post vaccination will be evaluated for the detection of antibodies.
Evaluate the immune response produced by rabbits using two different bacterial species, at different time intervals. To quantitatively determine an immune response, serum will be collected and measured at days 6 and 42 (post-vaccination) for Rabbit 1, day 42 (post vaccination) for rabbit 2. Still needs a bit more
I would explain more on the principles of agglutination, antigens and antibodies/antisera. (look for some papers on bacterial agglutination to help you with this part. It needs a little
The direct agglutination test (DAT) is a process by which the use of an antigen is an essential component for the detection of antibodies in serum. There are many different DATs available therefore the DAT we have selected is the microtitre agglutination technique.
Dilution is an important part of the process allowing us to determine the last point in which the activity can be detected. Thus, using a doubling solution to determine the end point will result in a semi-quantitative …show more content…
The epitope is part of the antigen that is recognised by the immune system, specifically by antibodies.
Extended Learning:
Antisera evaluation: bacterial agglutination.
Introduction:
The Direct Agglutination Test (DAT) For over 80 years, subtyping of Salmonella enterica by serotyping has been a standard practise, a process in which surface antigens are recognised through agglutination reaction (2). Global health issues among developing countries
Prevalence in Developing Countries:
Future Research:
Conclusion:
This study provides updated information on enteric bacteria diversity and antibiotic resistance in the Sahel region, where such data are scarce. Whether they are or not the causative agent of diarrhea, bacterial infections and their antibiotic resistance profiles should be closely moni-tored in countries like Niger where childhood malnutrition pre-disposes to severe and invasive infections (ref 6, not paraphrased).
This study produced up to date information, on data that would normally be difficult to attain due to ?
Introduction and Aim:
Agglutination is coalescing of antigen bearing cells, microorganisms or particles in the presence of specific antibodies, in a suspension. Antiserum is a serum containing antibodies which are specific for one or multiple antigens. Direct agglutination, a process by which antigens detect and bind directly to specific antibodies in serum against a microbe. Hence, the aim of this experiment is to qualitatively and semi-quantitatively analyse antisera by using direct bacterial agglutination techniques. The experiment will further determine the titre of antisera and the specificity of antibodies produced against two antigens (bacteria): Brucella abortus (B. abortus) and Brucella …show more content…
Involving the vaccination of proven naïve rabbits with an antigen enabling an immune response brought about by the rabbit identifying this anti-gen as foreign. Blood collection at various intervals post vaccination will be evaluated for the detection of antibodies.
Evaluate the immune response produced by rabbits using two different bacterial species, at different time intervals. To quantitatively determine an immune response, serum will be collected and measured at days 6 and 42 (post-vaccination) for Rabbit 1, day 42 (post vaccination) for rabbit 2. Still needs a bit more
I would explain more on the principles of agglutination, antigens and antibodies/antisera. (look for some papers on bacterial agglutination to help you with this part. It needs a little
The direct agglutination test (DAT) is a process by which the use of an antigen is an essential component for the detection of antibodies in serum. There are many different DATs available therefore the DAT we have selected is the microtitre agglutination technique.
Dilution is an important part of the process allowing us to determine the last point in which the activity can be detected. Thus, using a doubling solution to determine the end point will result in a semi-quantitative …show more content…
The epitope is part of the antigen that is recognised by the immune system, specifically by antibodies.
Extended Learning:
Antisera evaluation: bacterial agglutination.
Introduction:
The Direct Agglutination Test (DAT) For over 80 years, subtyping of Salmonella enterica by serotyping has been a standard practise, a process in which surface antigens are recognised through agglutination reaction (2). Global health issues among developing countries
Prevalence in Developing Countries:
Future Research:
Conclusion:
This study provides updated information on enteric bacteria diversity and antibiotic resistance in the Sahel region, where such data are scarce. Whether they are or not the causative agent of diarrhea, bacterial infections and their antibiotic resistance profiles should be closely moni-tored in countries like Niger where childhood malnutrition pre-disposes to severe and invasive infections (ref 6, not paraphrased).
This study produced up to date information, on data that would normally be difficult to attain due to ?