Antimicrobial Susceptibility, Mic and Mbc Essay

2200 Words Jul 26th, 2013 9 Pages
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Antimicrobial Susceptibility, MIC and MBC
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Microbiology Lab Report 3
Carolina Toscanojara

Antibiotic Sensitivity.

Objective:
Antibiotics are chemical compounds that selectively interfere with the growth of microorganisms while causing little or no damage to the animal or human host. The purpose of this lab was to determine the effectiveness antibiotics against any given bacterium by means of examining the zone of inhibition, the minimum inhibitory concentration, and the minimum bactericidal concentration.

Introduction: Chemical antimicrobial agents are chemical compounds that are capable of either preventing the microorganisms for
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The most suitable antibiotics are chosen for a Minimum Bactericidal Concentration (MBC), which is a basic quantitative test that determines the lowest concentration of such antibiotic required to kill 99.9% of the original inoculum in a given time. (Gene, 2010) Antibiotic resistance is nowadays one of the world's most persistent public health problems, about 70 percent of the bacteria that cause infections in hospitals are resistant to at least one of the drugs most commonly used for treatment. Some organisms are resistant to all approved antibiotics and can only be treated with experimental and potentially toxic drugs (Todar, 2008). Although not all antibiotic are necessarily killing bacteria, the smart use of them can help to control the spread of resistance. Antibiotic resistance is becoming a significant problem, as the number of resistant bacterial genera increase. It is known that there are bacterial strains that are resistant to all known antibiotics. For this reason, it is important to be able to determine the susceptibility of a microorganism to particular antibiotics so that the most appropriate treatment can be given to treat the infection.
Part of describing the function of an antibiotic is stating whether it is effective against Gram-positive bacteria, Gram-negative bacteria or both (broad spectrum

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