Antibiotic Resistance At An Shocking Rate ( Mezger Et Al Essay

1638 Words Nov 18th, 2015 null Page
Over prescription and extensive use of antibiotics has caused antibiotic resistance at an shocking rate (Mezger et al.2014). The main aims of Antibiotic sensitivity testing is detecting possible drug resistance in common pathogens by in-vitro testing of bacterial cultures with antibiotics to determine susceptibility of bacteria to antibiotic therapy. The tests are frequently used when the etiologic agents are members of species capable of resistance to commonly prescribed drugs (Jenkins and Schuetz,2012). The most-widely used tests in clinical laboratories include Manual: Broth Microdilution, Disc Diffusion and E-test. Automated instruments: MicroScan walkaway, Vitek2 system and PhoenixBD tests are commercially used.
Two manual tests used in laboratories are Disc Diffusion test and Broth Dilution. Although there are many other manual tests that can be used such as Stokes test also the agar dilution method which is very similar to the Broth dilution method and E-test (Lee et al.,2013).
Disk Diffusion test:
Disk diffusion is commonly it allows simultaneous tests to be held and completed effectively (Lee and Chung,2015). The test uses a Mueller-Hinton agar plate; known to be excellent for susceptibility testing of nonfastidious bacteria. Roughly 1–2×108CFU/mL of the bacterial inoculum is spread over agar plate surface, with a sterile cotton-tipped swab to form an even lawn. On the inoculated agar surface 12 pre-prepared, permanent concentration, paper…

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