Advances in biology have led to many life altering medications, treatments, and solutions to molecular issues. One such region of biology that has altered scientists’ stances on creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase.
The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it creates a palindrome from the overhanging sequence called a sticky end. Restriction enzymes can make one of two ends when creating recombinant DNA: sticky ends or blunt ends. When a gene is to be inserted into a vector or the new DNA sequence the restriction enzymes must cut sticky …show more content…
Three types of recombinant vaccines have been developed: live genetically modified organisms, recombinant inactivated vaccines, and genetic vaccines. Live genetically modified vaccines are viruses with part of the genome inactivated or knocked out. They can also be used as vectors or as infectious clones of a disease agent. Recombinant inactivated vaccines only contain part of a whole organism while genetic vaccines are recombinant plasmids made from purified bacteria. These recombinant vaccines are used for humans, swine, poultry, and sheep. Point mutations can also be induced by the size, location, and type of insertion or deletion in recombinant DNA. Recent developments have shown that overlapping DNA fragments using in vitro reactions result in the joining of DNA fragments at the termini. These site-directed mutagenesis involves the remodeling of the Polymerase Chain Reaction using mutagenesis primers in recombineering
The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it creates a palindrome from the overhanging sequence called a sticky end. Restriction enzymes can make one of two ends when creating recombinant DNA: sticky ends or blunt ends. When a gene is to be inserted into a vector or the new DNA sequence the restriction enzymes must cut sticky …show more content…
Three types of recombinant vaccines have been developed: live genetically modified organisms, recombinant inactivated vaccines, and genetic vaccines. Live genetically modified vaccines are viruses with part of the genome inactivated or knocked out. They can also be used as vectors or as infectious clones of a disease agent. Recombinant inactivated vaccines only contain part of a whole organism while genetic vaccines are recombinant plasmids made from purified bacteria. These recombinant vaccines are used for humans, swine, poultry, and sheep. Point mutations can also be induced by the size, location, and type of insertion or deletion in recombinant DNA. Recent developments have shown that overlapping DNA fragments using in vitro reactions result in the joining of DNA fragments at the termini. These site-directed mutagenesis involves the remodeling of the Polymerase Chain Reaction using mutagenesis primers in recombineering