Annotated Bibliography On Recombinant Dna Essay

1608 Words Jan 22nd, 2016 7 Pages
Advances in biology have led to many life altering medications, treatments, and solutions to molecular issues. One such region of biology that has altered scientists’ stances on creating a perfect product is the research being done on recombinant DNA. Recombinant DNA is any single molecule containing DNA sequences from two or more organisms. The process of creating recombinant DNA relies on the use of restriction enzymes, gel electrophoresis, and DNA ligase.
The first step in creating this new DNA strand is to cut the gene sequences you want from the original DNA molecule. This sequence will be cut by the restriction enzyme in such a way that it creates a palindrome from the overhanging sequence called a sticky end. Restriction enzymes can make one of two ends when creating recombinant DNA: sticky ends or blunt ends. When a gene is to be inserted into a vector or the new DNA sequence the restriction enzymes must cut sticky ends to create the hydrogen bonds that connect two sticky ends to make a recombinant molecule. A blunt end is used to terminate a sequence and create an endpoint. Restriction enzymes essentially act as the scissors of the cell to cut up foreign DNA into ineffective pieces (the cell’s DNA use methyl tags to protect themselves) or create strategic incisions to make recombinant DNA. Because of the length in bp of DNA, and the hundreds of restriction enzymes that have been recently found, scientists have been using restriction digests more and more in the…

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