Analysis Of Sanger And Next Generation Sequencing

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Biology is a huge subject with many sub branches, application and tools that have helped in the improvement of humanity. One of the many Biology branches, that is currently helping in medicine and other areas related to health, and biology, is the Bioinformatics. The way it is doing it is by improvement and be able to sequencing DNA (genome). Bioinformatics can be describes as a science tool in which use computer technology to analyze, collect, “storage, and retrieval of genomic data” (American Heritage Dictionary). There are different ways to sequence the genome or DNA, store and evaluate the information obtained. Some of the methods are Sanger and Next Generation Sequencing, also known as hierarchical and whole-genome shotgun sequencing. …show more content…
Making it a long process that produces 700 base pairs (bp) in an hour. In the other hand, Next Generation involves shorter steps, it amplifies the genome fragment by PCR and then determine the sequence by observing the addition of nucleotides as it is synthesized from the template. Also, most of the steps are performed by machine. A major difference between Sanger and Next Generation Sequencing is the read length produce, Next Generation produces 75 to 400 bp between 7 hours to 10 days and there is more analysis to do on Next Generation than Sanger. This technology is comprised of various platforms which include nanopore technology, Illumina and Roche/454. More genome data are now being produced by Next gene sequencing than Sanger DNA sequencing. (mention Any …show more content…
Epigenetics “refers to external modifications to DNA that turn genes "on" or "off." These modifications do not change the DNA sequence, but instead, they affect how cells "read" genes.” () Gene expression can be affected by the environment and the life style of the organism. Exploring the genome of different kinds of Drosophila can gave a better understanding of it changes or evolution of the dot chromosomes. Since Sager is an expensive tools and Roche/454 has more coverage but creates mononucleotide runs, also known as homopolymer runs, a hybrid assembly was made to our, and GEP, projects. The hybrid assembly, which will be our sequence, consists of data from Roche/454 and

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