Comparative Analysis Of Hormone And Hydrazone Carbamate

The % relative standard deviation (% RSD) was found to be below 5 % for all the four analytes in system precision and below 6 % in method precision. The data as shown in Table 3.4.3.3.3 and 3.4.3.3.4. LOD values observed for Hydrazine carbamate and Hydrazone carbamate were 0.0018 µg per mL and 0.00092 µg per mL respectively. The LOD value observed for both 1-Hydroxy benzotriazole and 2R, 3S Chlorohydrine hydrochloride were 0.0019 µg per mL. The LOQ values observed for all four analytes were 0.0037 µg per mL. The data as shown in Table 3.4.3.3.5 and 3.4.3.3.6.

Table 3.4.3.3.3 System precision for Hydrazine carbamate, Hydrazone carbamate, 1-Hydroxy benzotriazole and 2R, 3S Chlorohydrine hydrochloride

Area Counts Inj # 2R, 3S Chlorohydrine …show more content…
Hydrazine carbamate Hydrazone carbamate LOQ LOD LOQ LOD µg/mL 0.0037 0.0018 0.0037 0.00092 ppm or µg/g 0.366 0.183 0.367 0.092
Injection No. Area counts
1 2053 1255 2005 …show more content…
Area counts % Difference
Initial 31838 -
62 32307 1.47
124 33247 4.43
186 33198 4.27
249 33792 6.14
311 32268 1.35
373 31960 0.38
435 33777 6.09
497 33701 5.85
559 32775 2.94
621 30982 2.69
684 32473 1.99
746 31998 0.50

Table 3.4.3.3.12 Stability of analytical solution for Hydrazone carbamate

Time
(min.) Hydrazone carbamate Area counts % Difference
Initial 41210 -
62 40569 1.56
124 40671 1.31
186 40138 2.60
249 38130 7.47
311 38875 5.67
373 37567 8.84
435 37634 8.68
497 39788 3.45
559 38115 7.51
621 38900 5.61
684 36787 10.73
746 37659 8.62

Table 3.4.3.3.13 Stability of analytical solution for 1-Hydroxy benzotriazole

Time
(min.) 1-Hydroxy benzotriazole Area counts % Difference
Initial 33054 -
62 34041 2.99
124 35031 5.98
186 31993 3.21
249 33995 2.85
311 34017 2.91
373 36054 9.08
435 35459 7.28
497 33442 1.17
559 33143 0.27
621 33372 0.96
684 34388 4.04
746 34816 5.33

Table 3.4.3.3.14 Stability of analytical solution for 2R, 3S Chlorohydrine hydrochloride

Time
(min.) 2R, 3S Chlorohydrine hydrochloride Area counts % Difference
Initial 48667 -
62 48662 0.01
124 46742 3.96
186 48199 0.96
249 46748 3.94
311 48154 1.05
373 47559 2.28
435 48013 1.34
497 47874 1.63
559 48599 0.14
621 46848 3.74
684 48037 1.29
746 49232