In order to evaluate the efficiency of RAPD-PCR fingerprinting for Cajanus cajan L. cultivar, the DNA templates for 7 RAPD primers banding modality were used. The total number of magnified fragments in the Cajanus cajan L. cultivar value of each primer was represented in Table 2. Total of 18 bands were obtained for all primers. The size of the amplified bands was ranged between 1000 bp and 300 bp. Primers OP-A5, OP-B4, OP-B9, OP-C5 and OP-E15 showed at least three specific fragments. Primer OP-C5 showed unique fragment at ≈ 820 bp (Table 2). The RAPD banding obtained with the seven RAPD primers was presented in Figure 1a.
The banding modality of ISSR-PCR fragments using five qualitative primers to fingerprint the Cajanus cajan L. cultivar was documented in Table 2. The results of ISSR fingerprint exhibited 14 amplified fragments. The highest band mass was made with primer HB9, and the lowest band size was produced by primer B89. The size of the amplified fragments was ranged between 830 bp and 240 bp in length. The ISSR banding pattern obtained with the five primers was shown in Figure 1b.
3.2. Cell viability …show more content…
The FFCC at 200mg/kg b.wt did not affect the cell viability (93.8±1.39%) of mice bone-marrow cells. In contrary, CP extensively reduced the cell viability to 64.07±2.12% in comparison with 96.4±0.81% for non-treated cells. The serial administration of variable concentrations of FFCC concurrently with CP triggered reduction of cytotoxicity induced by CP alone. The percentages of cell viability increased significantly from 72.58±1.5% to 83.16±1.73% when animals simultaneously treated with CP and FFCC at 50 or 200 mg/kg b.wt respectively in comparison with CP alone (Figure
The banding modality of ISSR-PCR fragments using five qualitative primers to fingerprint the Cajanus cajan L. cultivar was documented in Table 2. The results of ISSR fingerprint exhibited 14 amplified fragments. The highest band mass was made with primer HB9, and the lowest band size was produced by primer B89. The size of the amplified fragments was ranged between 830 bp and 240 bp in length. The ISSR banding pattern obtained with the five primers was shown in Figure 1b.
3.2. Cell viability …show more content…
The FFCC at 200mg/kg b.wt did not affect the cell viability (93.8±1.39%) of mice bone-marrow cells. In contrary, CP extensively reduced the cell viability to 64.07±2.12% in comparison with 96.4±0.81% for non-treated cells. The serial administration of variable concentrations of FFCC concurrently with CP triggered reduction of cytotoxicity induced by CP alone. The percentages of cell viability increased significantly from 72.58±1.5% to 83.16±1.73% when animals simultaneously treated with CP and FFCC at 50 or 200 mg/kg b.wt respectively in comparison with CP alone (Figure