Analysis Of Apium Graveolens Extract
0.5ml of platelet poor plasma (PPP) will be obtained within 30 min to avoid the leakage of cellular components. Three aliquots consisting 50uL of PPP aliquot will be separated, 50uL of the extract with three different concentrations will be mixed and another 100uL of PPP aliquot will be the reference control. The aliquots will be incubated at 37 degrees Celsius for 10 and 3 minutes respectively. 200 ml of the reagent that has been incubated will be added to the aliquots; subsequently the timer will be started. The tubes will be constantly mixed as soon as there is formation of gel, the timer will be stopped and it will be recorded. The procedure will be repeated for three trials (Angeles et. al., 2013)
Microscopic Examination Wedge smears diluted with the extract, stained with Wright’s stain, will be viewed under a microscope to examine the preservation of cell size and shape. It will be compared with the smears made from the EDTA tube as the reference control.
5.5.1 Statistical Analysis The data gathered will be analyed using Analysis of Variance (ANOVA). This test will determine whether the different concentrations of the extract has a significant difference between the variables of the experiment.
5.6 Data Analysis
Prothrombin Time | Sample 1 | Sample 2 | 10% Extract | 13.4 s | 12.9 s | 13 s | 9s | 10s | 12.2s | 30% Extract | 11.1s | 14.6s | 11.1 s | 13s |