Analysis Of Anti-C Alloantibodies

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Anti-C alloantibodies are the most probable antibody present in the patient’s sample. Reagent screening cells 2 and 3 were not reactive at immediate spin, 37°C, or in the AHG phase. The screen ruled out antibodies to D, c, E, e, k, Kpb, Jsb, Fyb, Jka, Jkb, Lea, Leb, P1, M, N, S, s, Lub, and Xg*a antigens. In all positive screening cells, reactions occurred at immediate spin, 37°C, and AHG phases; however, screening cells with positive reactions did not react with same strength.
The panel ruled out antigens V*, Cw, K, Fya, and Lua. In panel cells, reactions occurred at immediate spin, 37°C, and AHG phases. This indicates the presence of IgG class antibodies, as IgM class antibodies only react at immediate spin. The C antigen was expressed in panel cells 1, 2, 5, 9, and 10 and weakly expressed in panel 11. Panel cells 1,2, 5, 9, and 10 reacted with patient’ serum. Panel cells 3, 4, 6, 7, 8, 11, 12, 13, 14, 15, and 16 did not react with the patient’s serum. With the
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None of QC reagents were expired, which is important because expression of antigens may decrease during storage. Quality control passed for QC1, QC2, and QC3. Positive controls, QC1 and QC2, demonstrated hemagglutination while the negative control, QC3 did not demonstrate hemagglutination. Additionally, check cells added to non-reactive demonstrated agglutination, indicating that AHG was added to the tubes. This means that the non-reactive tubes are not false negatives caused by the lack of AHG. Patient cell auto control as not performed in student lab. Auto control is running the patient’s own red blood cells with their serum for the antibody screen and identification. It is presumed that auto control for this patient was non-reactive, indicating alloantibodies. A reactive auto control indicates the presence autoantibodies and in vitro hemolysis of patient red cells by their own antibodies in the form of a pinkish-red supernatant would be

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