Hplc Analysis

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4.3 High Performance Liquid Chromatography (HPLC) An HPLC analysis was carried out for the Fraction C2-7 for confirmation of the results obtained by TLC analysis. According to the HPLC chromatogram, there is only one peak was observed at the retention time of 4.510 minutes. Therefore, it can be concluded that there is only one compound presents in the Fraction C2-7. Basically, the first few small peaks in the chromatogram which is around 2-3 minutes are ‘noise’ from the injection that been made. Each peak represents a component present in the sample. The chromatogram of the Fraction C2-7 shows in Figure 4.1. Figure 4.1: Chromatogram of the Fraction C2-7

4.4 Antioxidant activities
4.4.1 DPPH Radical Scavenging Activity The antioxidant
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A calibration curve for the total phenolic content of Gallic acid with concentration of 20, 40, 60, 80 and 100 μg/mL shown in Figure 4.4 and the results were expressed as gallic acid equivalents (GAE)/g of samples. Figure 4.4: Standard curve of Gallic acid. From the standard curve, an equation was obtained from the straight line, where y = 0.0061x - 0.0871 (R2= 0.9898). Table 4.10 shows the absorbance value of the methanol crude extract and the Fraction C2-7 that was substituted into the equation and the TPC was …show more content…
As the fraction sample have undergoes the separation process through column chromatography, phenolic compound becomes separated and lesser in every fraction collected. Total phenolic content in plant extract depends on the polarity of the solvent used in the extraction process (Stankovic, 2011). The high solubility of phenols in a polar solvent will provides high concentration of these compounds in the extracts (Zhou and Yu, 2004; Mohsen and Ammar, 2009). Therefore, methanolic crude extract should contain more phenolic compounds as polar solvent is able to extract more compounds from the plant extract and this have proven that Musa acuminata leaves have the potential to be a good source of phenolic

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