Analysis And Determination Of Genotypes And The Frequency Of A Human Specific Alu Insertion

1149 Words Dec 10th, 2015 5 Pages
Analysis and Determination of Genotypes and the Frequency of a Human-Specific Alu insertion (PV92) on chromosome 16

The human genome is made up of approximately 6 billion base pairs distributed on 46 chromosomes. A genome is an organism 's complete set of deoxyribonucleic acid (DNA), a chemical compound that contains the genetic instructions needed to develop and direct the activities of every organism. ("Human Genome…”, 2013) However, 98% of our genome does not encode protein sequences and the remaining genome that actually codes for protein support different processes in our bodies such as cellular metabolism, reproduction and development. After the discovery of our genome, it has widely revolutionized different disciplines in science specially in the field of forensics, making it an ideal diagnostic tool in identifying relationships and identities. In this laboratory exercise, our goal is to analyze and compare genotypic frequencies among different individuals from three different college institutions across the U.S. We hypothesized that the expected values from bioserver.org’s Chi Square will support our observed values. Also, for our own group (LAHC Fall 2015) there will be an even distribution of genotypic frequency of homozygous (+/+), homozygous (-/-), and heterozygous (+/-) individuals. Moreover, the human DNA sample was obtained through our cheek cells simply by way of salt water mouth washing. Moreover, the cell sample needs to be lysed and purified from…

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