An Investigation Into the Effects of Temperature on Enzyme Action

1237 Words Dec 18th, 2010 5 Pages
An investigation into the effects of temperature on enzyme action
An enzyme is a biological catalyst that speeds up the rate of reaction in certain biological functions. They play a vital role in many aspects of human physiology and are necessary for the functioning of a number of systems, for example in the digestive system to help to break down food. All enzymes have a unique active site that can fit on to a particular molecular arrangement on a target substrate; a substance e.g. carbohydrate, protein, or fat, that the enzyme is designed to breakdown. There are a number of different enzymes in the human body; each type produced specifically to perform a certain role. Enzymes are not themselves destroyed in the reaction to break down a
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These included measurements from 20 degrees c, to 60 degrees c, in ten degree intervals and included a measurement at 0 degrees c, for comparison. The substrate used was protein from skimmed milk.

Method
A one per-cent solution of trypsin in sodium hydrogen carbonate was used to deliver a uniform dose in a favourable P.H. environment. A skimmed milk powder preparation of 5% in 95% purified water was used to mitigate undue discrepancy in the results. All equipment, that came into contact with the study mediums, was sterile e.g. test tubes, syringes etc.
A cross was marked with a marker pen, on the side of one test tube at the bottom and then 2ml of trypsin solution was then placed in a second. The two test tubes were then placed in one of a number of water baths, set at varying temperatures, for five minutes, in order to raise them (or lower) to a target temperature. The time was measured with a digital stop clock. After five minutes both samples were removed and the trypsin was poured into the tube containing the milk. The stop clock was re-started and ran until the trypsin had broken down enough of the milk protein to make the marker pen cross clearly visible across the width of the test tube. This process was repeated, by five experiment groups, until each group had a measurement at each required temperature. The results were then recorded on a data table.
All due health & safety precautions were followed including

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