The fungi Alternaria is a phyto-pathogen cause leaf blight disease to variety of plants. Alternaria alternata and Alternaria helianthi was isolated from Dalbergia sissoo L. and Helianthus annuus L. respectively. Test antagonists grow faster than the pathogen and produced inhibition zones thereby limiting the growth of the pathogen. These antagonistic interactions influence the incidence and severity of the disease caused by the pathogen. When the two Alternaria spp. were tested by dual culture method in combination with T. koningiopsis, the result recorded found that A. helianthi (79.26 %) was most susceptible and revealed high percent of inhibition of mycelia growth while A. alternata (62.38%) showed lowest percent inhibition of mycelia growth.
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For the production of non-volatiles, three discs of mycelia agar plugs (6 mm diameter) obtained from edges of 7 days old culture of T.koningiopsis were inoculated in 100 ml sterilized potato dextrose broth (PDB) in 250 ml conical flasks and incubated at 25 ± 1°C on a rotary shaker at 100 rpm for 10 days. The control conical flasks were inoculated with sterile PDA plugs respectively. After incubation, the culture was filtered through Millipore filter for removing spores for collecting non-volatile metabolites from T.koningiopsis. Collect the transparent supernatant containing non volatile metabolites. For poisoned food assay the liquid formed nonvolatile was added to molten PDA medium (at 40 ± 4°C) to obtain a final concentration of 10% (v/v). The medium was poured in Petri dishes at 20 ml per plate and inoculated with 5 mm mycelial plugs of the pathogens in the centre of the plates and incubated at 25 ± 2°C for 7 days or until the colony reached the plate edge in control plate. Triplicates were maintained for each treatment and radial growth of the pathogen was recorded by using formula of Vincent (1947) (Shaikh and Nasreen,
For the production of non-volatiles, three discs of mycelia agar plugs (6 mm diameter) obtained from edges of 7 days old culture of T.koningiopsis were inoculated in 100 ml sterilized potato dextrose broth (PDB) in 250 ml conical flasks and incubated at 25 ± 1°C on a rotary shaker at 100 rpm for 10 days. The control conical flasks were inoculated with sterile PDA plugs respectively. After incubation, the culture was filtered through Millipore filter for removing spores for collecting non-volatile metabolites from T.koningiopsis. Collect the transparent supernatant containing non volatile metabolites. For poisoned food assay the liquid formed nonvolatile was added to molten PDA medium (at 40 ± 4°C) to obtain a final concentration of 10% (v/v). The medium was poured in Petri dishes at 20 ml per plate and inoculated with 5 mm mycelial plugs of the pathogens in the centre of the plates and incubated at 25 ± 2°C for 7 days or until the colony reached the plate edge in control plate. Triplicates were maintained for each treatment and radial growth of the pathogen was recorded by using formula of Vincent (1947) (Shaikh and Nasreen,