Frist: Pre validation trails we need to examine all the available USP methods for Albuterol Sulfate by injecting standard and test sample that fit our sample preparation and target reasonable concentration. By doing that we can chose which method we would use for further evaluation.
Second: crossing overt the USP method to our sample testing:
1- Linearity: we need to have a linearity curve for that method, the curve must include the standard concentration equivalent to our sample concentration. The curve also should include a total of five levels (50%, 75%, 100%, 125%, and 150% of the target concentration “based on the sample preparations”)
2- Specificity: injecting sample , standard , standard spiked with 25% sample. Examine that there
Second: crossing overt the USP method to our sample testing:
1- Linearity: we need to have a linearity curve for that method, the curve must include the standard concentration equivalent to our sample concentration. The curve also should include a total of five levels (50%, 75%, 100%, 125%, and 150% of the target concentration “based on the sample preparations”)
2- Specificity: injecting sample , standard , standard spiked with 25% sample. Examine that there