Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a lab technique that is used to separate and identify DNA and RNA according to the size of the individual molecule fragments. It has many common uses in modern molecular biology such as separation of restriction enzymes, analysis of PCR results, the number of DNA in a sample, DNA sequencing, DNA finger printing, and forensic science.
The basis behind agarose gel electrophoresis is manipulating the highly negative charge held by DNA. When placed in an electric current, the negatively charged DNA will be drawn towards the positive charge. In the case of the agarose gel electrophoresis, an agarose gel, a polysaccharide matrix often used in separation techniques, (approximately 0.7-2% agarose) and buffer mixture acts

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