INTRODUCTION
When it comes to Biology, one of the most well known, and interesting topics within the science is DNA and genetics. Although DNA is typically used in agarose gel electrophoresis, it was not provided for this lab, so the use of three different dyes had to make do. A null hypothesis was created indicating that dyes with a lighter molecular weight would not pass through the agarose gel easier than those with a heavier molecular weight. Where as the alternate hypothesis stated that, dyes with a lighter molecular weight would pass through the agarose gel easier than those with a heavier molecular weight. Generally speaking, it was predicted that if dyes with a lighter molecular weight could pass farther through the agarose gel then, …show more content…
Before studying the over all movement of the dyes, it is important to acknowledge the different aspects of the electrophoresis process that are critical for understanding the results of this lab. Presented within the lab handout, important items such as the loaded wells, the positioning of the electrodes and the agarose gel are labelled (Dowding, 2018). Found below, the figure indicates how much of the the dye has moved from the loading wells throughout the agarose gel. To begin, the Crystal Violet dye, found in loading wells 1 and 4, moved the least in comparison to the other dyes throughout the gel and had a purple-blue colour to it. As for the Safranine, located in wells 2 and 6, moved the second furthest of the three individual dyes and had a bright red colour to it. Next, found in loading wells 3 and 7, Methylene Blue moved the farthest throughout the gel, and as no surprise show a blue colour. When it came to loading wells 4 and 8, a mixture of the three previously stated dyes were placed within the agarose gel. While the agarose gel was in the electrophoresis tank for 30 minutes, it began to appear that the previously mixed dye had begun to disperse into their separate dyes. For the most part, the dyes that were now separated, migrated relatively the same amount as the dyes on their own
When it comes to Biology, one of the most well known, and interesting topics within the science is DNA and genetics. Although DNA is typically used in agarose gel electrophoresis, it was not provided for this lab, so the use of three different dyes had to make do. A null hypothesis was created indicating that dyes with a lighter molecular weight would not pass through the agarose gel easier than those with a heavier molecular weight. Where as the alternate hypothesis stated that, dyes with a lighter molecular weight would pass through the agarose gel easier than those with a heavier molecular weight. Generally speaking, it was predicted that if dyes with a lighter molecular weight could pass farther through the agarose gel then, …show more content…
Before studying the over all movement of the dyes, it is important to acknowledge the different aspects of the electrophoresis process that are critical for understanding the results of this lab. Presented within the lab handout, important items such as the loaded wells, the positioning of the electrodes and the agarose gel are labelled (Dowding, 2018). Found below, the figure indicates how much of the the dye has moved from the loading wells throughout the agarose gel. To begin, the Crystal Violet dye, found in loading wells 1 and 4, moved the least in comparison to the other dyes throughout the gel and had a purple-blue colour to it. As for the Safranine, located in wells 2 and 6, moved the second furthest of the three individual dyes and had a bright red colour to it. Next, found in loading wells 3 and 7, Methylene Blue moved the farthest throughout the gel, and as no surprise show a blue colour. When it came to loading wells 4 and 8, a mixture of the three previously stated dyes were placed within the agarose gel. While the agarose gel was in the electrophoresis tank for 30 minutes, it began to appear that the previously mixed dye had begun to disperse into their separate dyes. For the most part, the dyes that were now separated, migrated relatively the same amount as the dyes on their own