3.1 The isolation of Aeromonas hydrophila
When using selective medium developed by Rimler and Shoots (1973), we obtained 95 bright yellow colour isolates with white edge which lead to the alleged as A. hydrophila. Of 95 isolates were further screened using SNI 7303 (2009) methods which produce 56 isolates. The isolates were further screened using Dorsch et al. (1994) and Cascón et al. (1996) method.
3.2 Bacterial DNA isolation and Identification.
DNA amplification using Dorsch method produced similar size amplicon to control sample A. hydrophila ATCC 7966. While Cascón method produces various amplicon sizes, some of them are similar to amplicon produced by control isolate. Cascón and Dorsch methods produce inconsistent result. For examples, if we use control isolate (A.hydrophila ATCC 7966) and six other isolates, all of six isolates (SfB, SfN, SfL, SfM, SfP, and PfKT9) produces 685 bp amplicon using Dorsch method. However, using Cascón method only two isolates (SfB and PfKT9) produced similar size to control amplicon. …show more content…
The identification and cannot be made by using by biochemical test such as SNI 7303 method, Cascon method, and Dorch method only. Even using all the three methods together, the result is not yet 100% accurate. Accuracy of SNI 7303 methods is 8.9 %. The accuracy Dorsch method is 31% and Cascon method is 50%. Using the three methods together, the accuracy is 71%. Using the combined methods the accuracy is closer to the 16S rDNA. The three common methods used to identify A. hydrophila, are not suitable to be used to identify A. hydrophila in Indonesia and probably in other tropical countries. This is probably because of Cascón (1996) and Dorsch (1994) used bacteria samples from temperate regions. Therefore, we need to develop a new simple method to identify A. hydrophila which is suitable for tropical
When using selective medium developed by Rimler and Shoots (1973), we obtained 95 bright yellow colour isolates with white edge which lead to the alleged as A. hydrophila. Of 95 isolates were further screened using SNI 7303 (2009) methods which produce 56 isolates. The isolates were further screened using Dorsch et al. (1994) and Cascón et al. (1996) method.
3.2 Bacterial DNA isolation and Identification.
DNA amplification using Dorsch method produced similar size amplicon to control sample A. hydrophila ATCC 7966. While Cascón method produces various amplicon sizes, some of them are similar to amplicon produced by control isolate. Cascón and Dorsch methods produce inconsistent result. For examples, if we use control isolate (A.hydrophila ATCC 7966) and six other isolates, all of six isolates (SfB, SfN, SfL, SfM, SfP, and PfKT9) produces 685 bp amplicon using Dorsch method. However, using Cascón method only two isolates (SfB and PfKT9) produced similar size to control amplicon. …show more content…
The identification and cannot be made by using by biochemical test such as SNI 7303 method, Cascon method, and Dorch method only. Even using all the three methods together, the result is not yet 100% accurate. Accuracy of SNI 7303 methods is 8.9 %. The accuracy Dorsch method is 31% and Cascon method is 50%. Using the three methods together, the accuracy is 71%. Using the combined methods the accuracy is closer to the 16S rDNA. The three common methods used to identify A. hydrophila, are not suitable to be used to identify A. hydrophila in Indonesia and probably in other tropical countries. This is probably because of Cascón (1996) and Dorsch (1994) used bacteria samples from temperate regions. Therefore, we need to develop a new simple method to identify A. hydrophila which is suitable for tropical