ABSTRACT
The reactivity of acid base cements forming hydroxyapatite (HA) such as, β-tricalcium phosphate (β-TCP) and dicalcium phosphate dehydrate (DCPD). Amorphous calcium phosphates, prepared by precipitation from supersaturated solutions, can also react to form apatitic cements since they are thermodynamic unstable with respect to HA and have a setting reaction more independent of particle size. Calcium phosphate cement containing an antibiotic can be used for filling bone defects and to ensure local antibiotherapy. Therefore, in the present chapter proposal, cement paste were prepared by combining cement liquids comprised of Na2HPO4 with cement powders. Gentamicin sulfate was also loaded on the cements and its in vitro release was evaluated over a period of time. The cement setting times were compared before and after drug addition. According to results, the initial and final setting times of samples increase after drug addition.
Keywords: Biphasic, Bone cement, Injectability, Gentamicin sulfate, Drug release, β-tricalcium phosphate, Hydroxyapatite, Mechanical properties, Porosity, Calcium phosphate cement
INTRODUCTION
Calcium phosphate materials are widely applied as a variety of bone substitute materials in dental and …show more content…
Gentamicin solution with the concentrations of 0.1%, 0.5% and 1.0% were prepared. The model drug solution concentrations were analyzed via UV-Visible Spectrophotometer (Hitachi UV U3010) at 290 nm peak absorbency to obtain the respective absorbance values. The wavelength at 290 nm was determined as the peak absorbency wavelength as maximum absorbency at this particular wavelength was observed. The equation of the linear curve shown in Table 1 and Figure 1 produced with the three points of different concentrations was used to quantify the concentrations of gentamicin in the withdrawn sample solutions. Drug release profiles for the different sets of implants up to 14 days were
The reactivity of acid base cements forming hydroxyapatite (HA) such as, β-tricalcium phosphate (β-TCP) and dicalcium phosphate dehydrate (DCPD). Amorphous calcium phosphates, prepared by precipitation from supersaturated solutions, can also react to form apatitic cements since they are thermodynamic unstable with respect to HA and have a setting reaction more independent of particle size. Calcium phosphate cement containing an antibiotic can be used for filling bone defects and to ensure local antibiotherapy. Therefore, in the present chapter proposal, cement paste were prepared by combining cement liquids comprised of Na2HPO4 with cement powders. Gentamicin sulfate was also loaded on the cements and its in vitro release was evaluated over a period of time. The cement setting times were compared before and after drug addition. According to results, the initial and final setting times of samples increase after drug addition.
Keywords: Biphasic, Bone cement, Injectability, Gentamicin sulfate, Drug release, β-tricalcium phosphate, Hydroxyapatite, Mechanical properties, Porosity, Calcium phosphate cement
INTRODUCTION
Calcium phosphate materials are widely applied as a variety of bone substitute materials in dental and …show more content…
Gentamicin solution with the concentrations of 0.1%, 0.5% and 1.0% were prepared. The model drug solution concentrations were analyzed via UV-Visible Spectrophotometer (Hitachi UV U3010) at 290 nm peak absorbency to obtain the respective absorbance values. The wavelength at 290 nm was determined as the peak absorbency wavelength as maximum absorbency at this particular wavelength was observed. The equation of the linear curve shown in Table 1 and Figure 1 produced with the three points of different concentrations was used to quantify the concentrations of gentamicin in the withdrawn sample solutions. Drug release profiles for the different sets of implants up to 14 days were