Advantages And Disadvantages Of Bishacrylamide Gels

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Register to read the introduction… These two polymers are made to react and polymerize with each other by means of a catalyzed reaction of TEMED with APS which produces sulfide free radicals that initiate the binding of acrylamide to bisacrylamide and so on repeatedly. The gel system itself is also composed of two strata which are the stacking gel and the resolving gel respectively. The stacking gel is the upper layer of gel where the protein sample is loaded into wells imprinted into the gel. This gel has a lower acrylamide concentration than that of the resolving gel and thus has larger pores, consequently the gel itself does not inhibit the movement of large peptides much which in turn lets them catch up with the smaller peptides before entering the resolving gel, which is the gel wherein the sample is actually segregated and classified into different bands roughly according to molecular weight after it travels through the gel after a certain amount of …show more content…
It is also preferred because of its charge neutrality upon setting as it does not interfere with the electrophoresis which is highly reliant on the sample and the surrounding’s charges within the system. Lastly, the gel itself is colorless and protein stains do not adhere to the gel so there will be minimal problems with unnecessary stains that aren’t from the staining …show more content…
The buffers also provide the ions where the electric current will pass through which is very important since the technique will not function well without a good conducting medium. The Cl- ions also act as a disturbance that avoids the proteins closest to the resolving gel to enter the gel first before stacking occurs, since if some proteins get a head start then the bands would be in inaccurate places along the gel during staining, and thus may be

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