2.5 grams of chitosan was added in 100 ml of 2% acetic acid solution. The added chitosan in acetic acid was dissolved by stirring it continuously. It was then poured into the petri plate and put in the incubator at 70°C for 48 hours. The 2N sodium hydroxide were put in dry films for about 30 minutes at ambient temperature. The wet film from the glass plate were peeled off using sterilized plastic spoon. It was then washed with distilled water for 4 times to rinse off NaOH solution. After washing, the films were dried at 40°C for 20 hours in the
