A Study On Using Enhanced Efficeincy Essay

1195 Words Nov 28th, 2016 5 Pages
In order to improve photolytic deprotection with enhanced efficeincy, several other photoremovable groups have been developed. Figure X.X.X shows o-nitrobenzyl protecting group together with other common photolabile protecting groups such as 1-(o-nitrophenyl)ethyl (NPE), 2-(o-nitrophenyl)-propyl (NPP), 6-nitroveratryloxycarbonyl (NVOC), 2,2-bis(2-nitrophenyl)ethoxycarbonyl (diNPEOC), 4-N-[2-(2-nitropehnyl)-propoxy]carbonyl (NPPOC), 1-(3-nitrodibenzofuran-1-yl)ethyl (NDBF), 6-nitropiperonyloxymethyl (NPOM), p-hydroxyphenacyl (pHP), 7-(diethylaminocoumarin-4-yl)methoxy carbonyl (DEACM), and 6-bromo-7-hydroxycoumarinyl-4-yl-methyl (Bhcmoc) and 6-bromo-7-methoxycoumarinyl-4-yl-methyl (Bmcmoc). A combination of NPOM and NDBF caging group strategy has enabled two-photon caged phosphoramidite for DNA synthesis (Lusic et al., 2010).

Protecting group Nucleobase
Protection site Deprotection conditions References o-nitrobenzyl (o-NB) C(N4)

T(O4) Aq sodium citrate buffer (pH 3.5)/t-BuOH Pyrex filterd light from mercury lamp Stutz and Pitsch 1999

Seio et al., 2016 (o-nitrophenyl)ethyl (o-NPE) N(O4) Walbert et al., 2001

2-(o-nitrophenyl)-propyl (NPP) T(O4)
G(O6) Mikat et al., 2007
Sieo et al., 2016

4-N-[2-(2-nitropehnyl)-propoxy]carbonyl (NPPOC) C(N4) UV irradiation at 290 nm Furuta et al., 2007 6-nitroveratryloxycarbonyl (NVOC) C(N4)

T(N3) UV irradiation at >300 nm Alvarez et al., 1999

Iwase et al., 2003…

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