In 1975 there was research done on the effects of inorganic salts on tissue permeability and exploration of the effects of inorganic salt on some plant membranes and notice the effect of stabilization or destabilization agent. (B.W.POOVAIAH AND A.CARL LEOPOLD) Beetroots were tested to see if there an alter change in the permeability of beets and in damaged beet. In the experiment they discovered that CaCl2 decreases leakage of betacyanin from the damage tissues. They use slices of beetroot suspended in water to measure the amount of betacanin leakage. The CaCl2 is added to one of the beetroot slices, which resulted to a decrease in the amount of pigment leaking out. This shows that the salt CaCl2 had a stabilizing effect. Other salts of monovalent ions K, Li and NA gave graded series of effects towards lessening of leakage (B.W. POOVAIAH2 AND A.CARL LEOPOLD). In this experiment they show that there are different types of salts that can prevent leakage or promote leakage but in this case, monovalent ion such as K, Li and Na gave effects towards lessening of leakage in tissues. In the end result Ca had prevented less leakage than the other salts use. This can relate to figure 3 and 4, that potassium ions are stronger than sodium ions since potassium and calcium are slightly …show more content…
When using the cuvettes in this experiment, some of the solution is left over at the bottom of the cuvette and cannot be taken out. This error could lower the concentration of the solutions, which can be shown in the poor fit, which is r2=0.57, of the trend line. In figure 4, a possible intrinsic error found in the fit for both the negative direct proportions, could be found in the lack of control of how much betacyanin pigments are in each beet cell. Even though the beet was cut in small equal ratios, there is no control of having equal amounts of cell in each beet or amounts of betacyanin pigments found in the tonoplast. In the process of cutting the beta vulgaris some of the cells got damage and amounts of betacyanin were leaked out. This could of possible lower the amount of betacyanin that could have been absorb in the