A Study On Low Concentration H Essay

1434 Words Nov 16th, 2016 6 Pages
This study shows that low concentration H. pylori can activate THP-1 cells. This study also shows that bacterial lysates and heat-killed bacteria are able to activate THP-1 cells. These results may imply that the activation of THP-1 cells by H. pylori do not necessarily require the presence of intact live bacteria, and that the components/mechanisms that are significantly responsible for this immune stimulation are heat-stable.
H. pylori lipopolysaccharides are shown to play a significant role in live H. pylori-mediated immune stimulation this study, as evidenced by the lower immune stimulatory properties of live H. pylori bacteria with altered LPS structure compared to the wild type. Lipopolysaccharides are highly heat-stable and are not destroyed under regular sterilising conditions (Magalhaes et al. 2007). Further, this study finds that live H. pylori LPS mutant lacking LPS O-antigen, outer core, and/or inner core had reduced immune stimulatory activities compared to the wild type. This result implies that H. pylori O-antigen, outer core, and/or inner core played role in live H. pylori-mediated immune stimulation. The ΔHP0159, ΔHP0479, ΔHP1191, and ΔHP1284 LPS mutant-derived lysates did not show lower immune stimulatory properties compared to the wild type lysates, implying that LPS O-antigen, outer core, and inner core do not play a significant role in bacterial lysate-mediated immune stimulation. Perez-Perez et al. (1995) found that purified H. pylori LPS could…

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