A Study On Dna Polymorphisms Essay

829 Words Feb 29th, 2016 4 Pages
The experiments covered in this report are based on the topic of DNA polymorphisms, these according to Houseman (1995) are differences in the DNA that occur between individuals. The polymorphisms under investigation here are classified as VNTR’s (variable number tandem repeats) and RFLP’s (restriction fragment length polymorphism). The first experiment is concerned with DNA polymorphisms that are known as VNTR’s, sections of DNA which have repeated nucleotide base sections in which the repeats vary in number per individual, here the VNTR’s chosen were D1S80 and D3S1358. The second centred on Restriction Fragment Length Polymorphism (RFLP) analysis and how the technique lends itself to the identification of genetic diseases. Each experiment uses the molecular technique PCR (polymerase chain reaction) in order to amplify the desired regions of DNA. PCR works by adding the DNA sample to synthetic DNA primers, DNA polymerase and nucleotide bases, then putting the mix through a number of PCR cycles. Each cycle undergoes a series of specific temperature changes with each change designed to move the amplification process to the next stage. The stages of one PCR cycle are denaturation, annealing and DNA polymerisation this causes the DNA fragment to multiply in numbers exponentially as shown by Hartl and Ruvolo (2012), meaning that 25 cycles of PCR leads to 225 (or 33,554,432) fragments being produced. Gel electrophoresis is the other molecular technique used in the…

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