A And B: Antibiotic Resistant Bacteria

Cells are the basic units of life. Each cell has different functions and properties that make life diverse. In this experiment, we had to deal with infections in two people. In order to treat the infections, we had to conduct a Grams’ test to figure out what kind of infection the children had and then identify the antibiotic that would best treat the infection or if they were antibiotic-resistant bacteria. For this experiment, my hypothesis is that both culture’s A and B will not be antibiotic-resistant and will have at least one antibiotic that will be able to treat the infections. The null hypothesis is that culture’s A and B will be antibiotic-resistant. In the experiment, we started off by sterilizing a loop over a Bunsen burner. Once sterilized, we got a drop of water using the loop and put it on a slide and sterilized the loop once more. Once sterilized, the neck of a culture tube was flamed and bacteria were collected from the agar in the culture tube. The test tube’s neck was flamed once again and the cap was replaced. The water and bacteria was mixed on the slide and allowed to air dry. Once air-dried, we passed the slide over the …show more content…
Mary Farmer has Gram-positive cocci, Streptococcus aureus, and Brian Evans has Gram-negative bacilli, Escherichia coli. From this, we know that Culture A’s bacteria has a thick cell wall with no outer membrane and Culture B has a thin cell wall with an outer membrane. From the results of the Gram’s test, neither patient is infected with more than one strain and based on the zones of inhibition in Table 5-2 of the lab manual, the antibiotics, Tetracycline and Chloramphenicol, can be used to treat both infections because they are broad-spectrum antibiotics. Broad-spectrum antibiotics are not desirable, therefore Nalidixic Acid or Streptomycin are best used to treat Mary Farmer’s infection and Kanamycin is best used to treat Brian Evans’