4.2.1 Test For Milk Lab Report

Decent Essays
3.4.2.1 Test for milk:-
1. Determination of SNF in milk
AIM: - to determine the SNF of milk
Apparatus: - lactometer jar, lactometer and thermometer
Principle: - It is calculated by using lactometer method.
Procedure:-
 Warm the milk sample to 40 to 45oCand maintain at this temperature till 5 min.
 Mix the contents by rotating and inverting the bottle, taking care to avoid the formation of air bubbles.
 Cool the sample to 15.5oC
 Invert the sample bottle 2 to 3 times; pour enough milk into the lactometer jar, do not let the air bubbles to form, so that some milk overflows when the lactometer is inserted.
 The lactometer should float freely and not touch the sides of cylinder.
 Allow the lactometer to remain steady in the milk. Take the
…show more content…
Determination of fat in milk
AIM: - To determine the fat content by using gerber method.
Principle: - The milk is mixed with sulphuric acid and iso-amyl alcohol in a special Gerber tube, permitting dissolution of the protein and release of fat. The tubes are centrifuged and the fat rising into the calibrated part of the tube is measured as a percentage of the fat content of the milk sample.
Apparatus: - Butyro meter, 10.75ml pipette,amyl alcohol, stoppers, gerber centrifuge, water bath.
Reagents:-Sulphuric acid, amyl alcohol.
Procedure:-
 Transfer 10 ml of sulphuric acid in to butyro meter .
 Warm the sample approximately to 270C and mix thoroughly but do not shake it so vigorously as to cause churning of the fat. Allow the sample stand for 3-4 min. to avoid air bubbles Invert the sample bottle 3-4 times immediately prior taking milk for test.
 Transfer 10.75ml sample into the butyro meter .
 Add 1ml of amyl alcohol and close the neck of butyro meter firmly with the stopper without disturbing the contents. Shake the butyrometer carefully and invert it 2-3 times so that the sample is thoroughly
…show more content…
Procedure for adulteration tests:-
A. Test for detection of Sugar test:-
Principle:- sugar was added to increase the density to prevent detecting of added water. Generally cane sugar is mixed in milk to increase the percentage solids content of milk i.e., to increase the lactometer reading of milk, that was already diluted with water.
 Take 10 ml of milk in a test tube. Add 5 ml of hydrochloric acid along with 0.1 g of resorcinol. Shake the test tube well and place it in a boiling water bath for 5 min. Appearance of red color indicates the presence of added cane sugar in milk.
 Dark brick red color indicated adulteration. (-ve) (+ve) Fig:- 2
B. Test for Detection Of Starch
Principle: - Starch was added to musk adulteration of milk with water to increase viscosity & lactometer reading. Addition of starch increases the SNF content of milk. Wheat flour, arrowroot, rice flour, etc., can also be added for increasing the SNF content.
 Take 5 ml milk in a test tube and boil it thoroughly. Cool and add a few drops of starch reagent. Change of color to blue indicates that the milk is adulterated with

Related Documents

  • Decent Essays

    Stir the solution and heat it until the solid dissolves. Now add 6.5 mL of HCl to this solution. Cool thoroughly in an ice-water bath. In a separate beaker, make up a solution of 3.7 g of sodium nitrite in 10 mL of water. Take a small beaker and weigh out about 5 g of ice and pour it into the conical flask having the reaction mixture.…

    • 2234 Words
    • 9 Pages
    Decent Essays
  • Great Essays

    Beta Vulgaris Experiment

    • 1512 Words
    • 7 Pages

    Put the discs on the white tile. 5. Using a 10cm3 measuring cylinder, measure 10cm3 of distilled water and pour it into a test tubes. Repeat this step for the other four test tubes 6. Label the test tubes with a different temperature each 7.…

    • 1512 Words
    • 7 Pages
    Great Essays
  • Superior Essays

    Elisa Research Paper

    • 1444 Words
    • 6 Pages

    Before putting it in the biosafety cabinet, spray the bottles and gloves with 70% ethanol. Then, suck out the substrate and dispense in the organic waste flask. Wash all the flasks with PBS to remove any residue of the substrate and then dispense the liquid. After that, add trypsin/EDTA to remove the cells from the bottom of the bottle. Incubate the bottles for 5 minutes.…

    • 1444 Words
    • 6 Pages
    Superior Essays
  • Improved Essays

    • The ideal temperature for frothed milk is 140 -155 degrees Fahrenheit. Place thermometer inside milk when frothing and pay close attention to the temperature of milk. • When using the steam wand to steam or froth milk, you must always purge the wand before preparing the…

    • 958 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    Homogenizing the milk is the next step, this decreases the milk fat to become smoother and creamier. The mix is then aged for four hours overnight at 40°F. This allows the mix to form crystals. Adding flavors should be done before freezing. This ensures that the mix will flow properly through the freezing equipment.…

    • 1309 Words
    • 5 Pages
    Great Essays
  • Decent Essays

    Lactose Lab Report

    • 445 Words
    • 2 Pages

    If glucose was present we labeled it with a “+” symbol and if it was negative we labeled it with a “-“ symbol. Results: Data Table for Lactase Enzyme Lab Test Tube #1: Milk + Enzyme solution at room temperature + Test Tube #2: Milk + Denatured enzyme solution - Test Tube #3: Milk + Water - Test Tube #4: Sucrose + Enzyme solution at room temperature - Test Tube #5: Sucrose + Denatured enzyme solution - Test Tube #6: Sucrose + Water - All of the solutions were in the test tube for duration of 2 minutes and then tested with the glucose stick being placed in the solution for duration of 30seconds. Below you will find the…

    • 445 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Place a thermometer in the beaker of water and check the temperature. Make sure the water is at 20°C. 3. Select a solvent to test for solubility first; for the method, sugar (sucrose) will be tested first. 4.…

    • 1650 Words
    • 7 Pages
    Improved Essays
  • Improved Essays

    It has the energy to detoxify your blood and kidneys to properly get rid of waste material from your body. Ingredients 1/2 teaspoon of baking soda (2 g) 1 cup of water (200 ml) How do I use it? Add the 1/2 teaspoon of baking soda to a cup of water and drink it on an empty stomach, without any other food. Repeat for three straight weeks. Note: Don’t consume more than the recommended amount or you could over-alkalinize your body.…

    • 757 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Salicylamide and NaI were dissolved in ethanol and cooled in a water bath. Then household bleach was added while stirring vigorously. Then NaOCl and HCl were added as well. Product was collected by vacuum filtration and then recrystallized from 96% ethanol and obtained a yield of 2,07g (54%). IR- and H-NMR-spectrum were taken.…

    • 960 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    3. Add 1 mL of the alkaline-potassium iodide-sodium azide solution at the surface of the liquid. 4. Replace the stopper, avoid trapping air bubbles and shake well by inverting the bottle several times. Repeat shaking after floc has settled halfway.…

    • 4915 Words
    • 20 Pages
    Great Essays