10% GelMA was synthesized by weighting 500 mg GelMA and 25 mg Photoinitiator (PI), which dissolved in 5 ml of PBS. The mixture was vortex and incubated at 80◦ C in the oven for an hour. The discs performed by adding 80 µL of the mixture into each well in the mold. The mold exposed to UV light, which set at 800 mw and 8.5 cm height for 60 seconds. Similar protocol has been used for synthesis 20% PEG discs. Weighted 1000 mg of Poly Ethylene Glycol (PEG) and 25 mg PI that dissolved in 5 ml of PBS. Vortex the mixture and incubated at 80◦ C in the oven for 30 minutes. 80 µL of the mixture was added into the mold and exposed to UV light at same condition of GelMA preparation. All discs were preserved in PBS solution in refrigerator,
