An inverted fluorescence microscope is an inverted compound light microscope equipped with modules to internally separate the different wavelengths of light, and direct them to respective detection channels. Its main components include a light source, an objective, a filter cube and a detection unit, which can be an eyepiece or a camera (Fig.1). Filter cube is a component which separates a fluorescence microscope from a light microscope. It consists of one excitation filter, one emission filter…
This artifact is primarily based on Sinusoidal waves and how to use and apply this to represent and develop musical notes. In doing so, I had to use a variety of properties of trigonometry for the key aspects in this artifact. Such as, building an instrument and finding out the relationship between sound waves and the sinusoidal representation. Sinusoidal is any graph mainly consisted of either the sine or cosine function. The general formula for Sinusoidal waves is y=Acosfx+C.The height of the…
Fresnel Equations The intensity of reflection in between two different medium of propagation can be calculated by using Fresnel’s equation. Light can either be s-polarized, p-polarized or unpolarized. The light radiated by the sun is an unpolarized light. In order to calculate the total reflection of an unpolarized light, we must first solve the reflected light using the Fresnel equation for s and p polarized reflection. For simplification purpose, the derivation of Fresnel’s equation will be…
order because of their wavelengths, which vary from 400 nm (nanometre or 10-9 m) to 700 nm as shown in the electromagnetic spectrum. In a primary rainbow, which is when light reflects a single time within the water droplet, red appears at the top of the rainbow because it has the longest wavelength, which is 700 nm, and is refracted the least when white light passes through the water droplets. Violet appears at the bottom of the rainbow because it has the shortest wavelength, which is 400 nm,…
A wave is a disturbance of energy. Sound and light are both disturbances in energy, and they both travel in the forms of waves. While sound travels in a longitudinal wave, light travels through a transverse wave. From a perspective of a human, sound and light on earth is very different compared to sound and light in space. These waves have differences in speed, form, shape, and other properties. Despite many of the differences, sound and light are very similar to each other. Light is a…
Photon detection efficiency (PDE) refers to the probability that a photon arriving on the SiPM surface is detected, an initiates the process of current pulse generation. PDE is a function of the overvoltage ΔV across the terminals of the APD and wavelength λ of the incident photon. Photon detection efficiency is one of the most important characteristics of a SiPM. PDE is the product of three factors :- Geometrical fill factor (FF), Quantum Efficiency (QE) and Probability of a Geiger discharge.…
re-describing phase) In order to students to understand the property of light that light consist different wavelength. Firstly we recapped the knowledge which we were previously discovered and the prism experiment they did in the high school. From the results they obtained in the filter experiment plus the previous knowledge, with our assistance, they eventually understand that light consists different wavelength. According to the inquiry framework for science teaching, the last thing we did is…
When looking at a flashlight, it can be determined that the flashlight itself resembles a parabola. Flashlights are supposed to be able to focus on a single point, which is infinitely small. Since a flashlight is supposed to mirror a parabola, the flashlight has similar components as a parabola. The filament, which is the part of the flashlight that gives light, resembles the focus on a parabola. The filament is a three dimensional object so it is infinitely larger than a point, yet the filament…
When a lens produces an image, the size of it corresponds to the focal length of the lens. The smaller the focal length, the smaller the image and the longer the focal length the bigger the image. How bright an image may be solely depends on the amount of light collected by the objective lens. How much light can be collected corresponds to the area of the objective lens. So the bigger the objective lens, the more light can be collected. If you want to increase the light gathering power by 4, you…
undergoes various types of Propagation Effects like – Reflection, Diffraction and Scattering because of the presence of obstructions like buildings, mountains, hills etc. Reflection is when the EM waves encroach on objects that are greater than the wavelength…