Thin layer chromatography

Abstract: A simple, novel, and sensitive method developed for estimation of Dappagliflozin and Saxagliptin. The method was carried out on reverse- phase C18 inertsil column, with mixture of Methanol: Water ( 80:20) ratio as a mobile phase, 1 ml / min flow rate. Different analytical parameters such as linearity, precision, accuracy, Limit of detection (LOD), Limit of Quantification (LOQ), robustness were determined according to the International conference on Harmonization ICH guidelines. The…

Twenty Korean barley cultivars (Geongang, Gwangan, Nackyoung, Dahyang, Daejin, Saegang, Boanchal, Keunal, Saessal, Saechalssal, Sujung, and Boanchalssal) were selected in this research. All the cultivars were developed by the National Institute of Crop Science (NICS), Rural Development Administration (RDA), Korea and were grown during 2013 in the experimental fields of the NICS, Milyang, Gyeongnam. After harvesting, barley leaves were immediately washed with clean sterile water and then…

the sake of extraction, spinach is first dehydrated with 100% ethanol so that extraction is more efficient. It is then extracted using dichloromethane (DCM) and maceration. The chlorophyll and β-carotene pigments are dissolved into the organic DCM layer which is polar and does not mix with water. The solution is then washed with sodium chloride (NaCl) to draw out any remaining water left in the solution that was not dried out by the ethanol. [1] Water will move toward the more hypertonic…

skin patient histology was characterized by thickness of the epithelial layer of the skin, presence of vacuoles or holes and increasing in the inflammatory cells in the dermis layer (Figure 7 A) indicating skin lichenification and inflammation of the skin due to itching. On Contrary, the treated skin patient showed decreasing in the thickness of the epidermis and decrease the inflammatory cells infiltrate into the dermis layer (Figure 7 B). These improvements can be explained according to the…

Development and evaluation of an antimicrobial formulation containing the essential oil of Thymus fontanesii Boiss. & Reut . Mebarki Noudjoub , Nabiev Mohamed2, Chader Henni3, Ziane Henifa4, Fazouane Fethia1 1 Food Technology Research Laboratory, FSI, M’hamed Bougara University, Boumerdès 35000, Algeria 2 Department of Chemical and Pharmaceutical Process Engineering, FHC, M’hamed Bougara University, Boumerdès 35000, Algeria 3 National Laboratory of Control of Pharmaceuticals Products (NLCPP)…

Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

The concentration of CDCL and CSER is obtained by solving equation (1) and (2) CX = A1 / ax1 ………………..... (1) CY = [A2 – (ax2 × CX)] / ay2…...(2) Where,  A1 and A2are absorbance of mixture at λ1 and λ2respectively.  ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,  ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,  CXandCYare concentrations of DCL and SER, respectively. Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4)…

Column Chromatography is considered one of the main techniques of separation in addition to SDS-PAGE and gel electrophoresis. This method can be useful in both a small scale as in a laboratory experiment as well as in a larger scale at the industrial level. It is also applied in different fields ranging from biology and biochemistry to microbiology and medicine. Column chromatography is also referred to as gel filtration or size exclusion chromatography among many other terminologies and it is…

Previously, for the first part of the experiment, it was hypothesized that the retention times of the eluates were invariably associated with the polarity of the compound; the more non-polar the compound, the faster it would be eluted out of the gas chromatogram. It was also hypothesized that the retention times decreased as the temperature in the gas column increased. In the first part of the experiment the relationship between the temperature and retention time, and the non-polarity of the…

Protein Separation Using Gel Filtration Chromatography Introduction: Chromatography techniques are one of the most useful methods use for the isolation or separation of macromolecule from a mixture. The most common used chromatography techniques are thin -layer chromatography (TLC), column chromatography, etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture based on their size. Also, collecting elutes that run through…