however it could take up to an hour to see the color change. SIM test then performed using inoculating needle in semi-solid media contains casein, amino acids, iron-contain compound and sulfur in form of sodium thiosulfate. Inoculated by stabbing technique and incubated for 24 hours, if the organism could reduce sulfur, a black color would be seen. To test for indole production, four or five drops of Kovak’s reagent was added into the cultured, a red color indicated a positive result. Last was…
was positive for both acid and gas production and eliminated K. pneumoniae, and E. aerogenes because they can produce acid, but no gas production was suggested from the dichotomous key. The test result for SIM deep slant that was used to test for sulfur-reducing turned up positive due to its color changed into a blackening color. The test result eliminated P. aeruginosa and identified unknown #63 as being Citrobacter freundii. During the unknown lab project, I was quite thankful that there…
water soluble (mainly sulfur oxide) to the ground. The water cycle itself is not harmed by acid rain, as only the rain part is acidic. The acid is absorbed into the ground and becomes part of the ocean or soil. If the ocean becomes too acidic however, the cycle can become very acidic; as ocean evaporation would potentially contain acidic properties that would rain down again. Oxidation Sulfur dioxide and nitrogen oxides enter the atmosphere via emissions from the combustion of sulfur-containing…
the cup’s mass will not affect the masses of the objects the group was trying to measure. During this investigation, it was determined that there is a 0% change in mass after a chemical change in a closed system, because the mass of the copper and sulfur before heating did not change after being heated. Although the substances had changed in appearance and could no longer be considered…
The main causes of acid rain are coal fired power plants, and combustion in cars and other motor vehicles. When coal is burnt, the sulfur combines chemically with the oxygen to form sulfur dioxide which then dissolves in rainwater to form sulfuric acid, a highly corrosive and strong acid (BBC Bitesize - GCSE Chemistry - Products And Effects Of Combustion - Revision 3, 2017). In an engine’s combustion, the temperatures…
organic deposits derived from decaying plants and animals that have been fossilized over millions of years. There is more to them than that however they are also hydrocarbons comprised primarily of the following elements: carbon and hydrogen and some sulfur, nitrogen, oxygen, and minerals. Different combinations of these elements are used as a fuel for our everyday lives. This paper’s purpose is to explain some of the chemistry behind the use of these various fossil fuels as well as just some…
If an organism can reduce sulfur to hydrogen sulfide, the hydrogen sulfide will combine with the iron to form ferric sulfide, which is a black precipitate. My SIM turned black, so it indicates the reduction of sulfur. Next, Indole helps to determine the ability of the organism to convert tryptophan into the indole. Which resulted to have a red color on top. Next, the citrate…
Chlorine is a naturally occurring substance. Chlorine has proven to be useful in the sanitation of water and its viability as a disinfectant. However, its manipulation and use in forming artificial chemical products has had devastating effects on the environment. Chlorine plays a major role in environmental problems such as depletion of the ozone layer, global warming and acid rain. It is most common in salt, but it is also prominent as organochlorines in solvents, pesticides, plastics,…
isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a sulfur-reducing media. This media selects for sulfate reducing organisms. There are many tests that can determine if Desulfovibrio was isolated or not. One test that will be conducted is a gram stain test. This will determine if Desulfovibrio was…
Sulfur dioxide promotes heart disease and asthma. Coal-burning plants are industrial polluters States producing about one-third of our carbon dioxide (CO2, a major contributor to GLOBAL WARMING), 40% of our mercury (highly TOXIC if ingested), one-quarter of our nitrogen oxide (found in smog) and two-thirds of our sulfur dioxide (a part of acid rain). But yeah, let’s all continue using this source of energy because…