Sodium hydroxide

The purpose of these calculations is to identify the limiting reagent and calculate the percent yield of the esterification reaction used to produce aspirin. This reaction was catalyzed by concentrated H2SO4, but since it is not consumed during the reaction, it does not affect the stoichiometric calculations. In order to determine the limiting reagent, the moles of reactants were calculated (Part A). Part B of the calculations illustrates the amount of aspirin produced by each reactant based on…

Methods: To begin, our group acquired three premade vials of .1ml mammal blood with coagulant. We then labeled the vials numbers one, two, and three. In vial number one we added 1 ml of Stock 300 mM NaCl, and no distilled water. This resulted in a NaCl concentration of 1.8 percent. In the second vial we added .5 ml of Stock 300 mM of NaCl, and .5 ml of distilled water. This resulted in a NaCl concentration of .9 percent. In the third vial we did not add any Stock 300 mM of NaCl, and 1 ml…

A range of concentrations (1nM to 30μM) of acetylcholine (Ach) and 5-hydroxytryptamine (5-HT) was administered to organ bath containing rat ileum. Ach and 5-HT induced contractions from 3nM (Fig. 1) and 10nM (Fig. 2) onwards respectively, both generating a shallow slope at the beginning of the concentration-response curve. EC50 of Ach is 7.47 x 10-7M whereas EC50 of 5-HT is 7.97 x 10-7M. The contraction generated by Ach and 5-HT was expressed in % Emax. As shown in Fig. 1 and 2, % Emax…

Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…

At 20 seconds, pH = 7.35 2. At 40 seconds, pH = 7.29 3. At 60 seconds, pH = 7.24 4. Did the pH level of the blood change at all during this run? If so, how? Yes, the pH level of the blood decreased. 5. Was the pH level always within the “normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No, acidosis occurred at 40 seconds when the pH dropped out of “normal” range to…

The developed method consisting the mobile phase 3g of Docusate Sodium was dissolved in 580 ml of Methanol, sonicated to dissolve to this 420 mL of milli Q water was added and pH was adjusted at 3.0 with perchloric acid with isocratic programming, XTerra RP 18 column (250mm×4.6mm, 5μm) column as stationary phase with…

Experiment 1: Standardisation of approximately 0.25 M Hydrochloric Acid Abstract The aim of the study was to standardise a solution of hydrochloric acid with an approximate concentration of 2.5 M against sodium carbonate solution. The main steps of this experiment were preparing the sodium carbonate solution, transferring the solution into a conical flask by using a pipette, adding the bromophenol blue indicator and titrating with hydrochloric acid until the first green colour appeared. The…

Student number: s3333523 Email-address: lidiapalha@gmail.com Name of demonstrator: H.Helbert Reaction Equation Summary Salicylamide and sodium iodide are dissolved in ethanol, and stirred and cooled to 0. After that household bleach was added while stirring vigorously, solution changed from colorless to pale yellow. Sodium thiosulphate and hydrochloric acid were added aswell. Product collected by vacuum filtration and recrystallized from 96% ethanol. IR- and H-…

The GCMS analysis showed the metabolites could be definitely identified as concern compound by comparisons with known authentic compound. The m+ ion is found at m/z94 for phenol. The molecular weight of the phenol is 94. The base peak value of phenol was observed as 94 and mass peak values were recorded as 66, 39, and 55. The m+ ion for acetophenone is m/z 120. The molecular weight of the compound was observed as 120, the base peak and mass peak values were recorded as 105 and 91, 77, 51, 39,…

Discussion Graph 1 displays the reaction time (in minutes) of how long it took for the Alka-Seltzer tablet to completely dissolve per trial. Graph 2 displays the averages of the reaction time which showed the higher the water temperature is, the faster the reaction time would be. At 65⁰C the average time for the Alka-Seltzer to completely dissolve was 1 minute 4 seconds followed by 1 minute 20 seconds at 35⁰C and ending with the highest average at 2 minutes 1 second (1.61). The hypothesis…