Restriction fragment length polymorphism

intragenic recombination may be occurred in the recombination hotspots or cold spots (Mourad et al. 1994). Tto identify the intragenicthis recombination, restriction fragment length polymorphism (RFLP) markers within target genes were used to distinct two parental alleles in traditional genetics . The intragenic recombination causes a distinct fragment pattern from both parents (Okagaki and Weil 1997). To easily discover the intragenic recombination, two individuals carried non-functional…

Picture this: A male forensic scientist just received a call to examine a crime scene with a dead body, possible homicide. At arrival, he discovered variations of individual evidence—some of which included multiple sets of latent fingerprints and a blood spatters—that could lead to a possible suspect or even the culprit responsible. Immediately, the forensic specialist begins to powder, lift, and collect the evidence. Considering the value of the individual evidence found—as D. P. Lyle stated in…

before adding ethidium bromide which will stain the DNA fragments as they move through the gel. After mixing the agarose and bromide, we constructed the chamber and proceeded to carefully pour the agarose into the chamber. After pouring the agarose, we proceeded to add 1X TBE buffer to the chamber. To load and run Gel 1, we added 6µl of 10X Blue Juice to our PV92 tube. We then obtained a tube of DNA size markers which contained known DNA fragments of 766, 500, 300, 150, and 50 base pairs which…

DNA Fingerprinting Introduction Background: DNA fingerprinting is one of the great discoveries of the late 20th century,that has revolutionized forensic investigations. (Roewer 2013) The complete DNA of each individual is unique, with the exception of identical twins. It is this difference that can be used by forensic scientists to match specimens of blood , tissue, or hair follicles to an individual with a high level of certainty. A DNA fingerprint, therefore, is a DNA pattern that has a…

Preclinical points of view Metformin improves the insulin resistance primarily in muscle, liver, and adipose tissues by reducing the liver glucose output, largely due to a diminution in the rate of glycogenolysis and gluconeogenesis (97). Various beneficial effects of metformin include attenuation of abnormal glucose metabolism, weight loss, improvement of insulin resistance, endothelial dysfunction, subclinical inflammation, lipid-lowering properties, antineoplastic potential and cardiovascular…

chain of custody, this has to be followed every time the evidence is moved or examined. Most labs use restriction fragment length polymorphism or RFLP to analyze DNA.RFLP requires investigators to dissolve the DNA in an enzyme that breaks the strand at specific points. The number of repeats affects the length of each resulting strand of DNA; investigators can then compare samples by comparing the lengths of the strands. Once the DNA in question has been amplified a short tandem repeat (STR)…

Introduction: The definition of Genetically Modified Organisms in the Britannica Encyclopedia is given as “Genetically Modified Organisms (GMO) is the organisms whose genome has been engineered in the laboratory in order to favor the expression of the desired physiological traits or the production of the desired biological products”. From the definition we can understand that the genome from the DNA can be altered or some sequence can be separated from the DNA and use it to enhance or mute (gene…

had two visible bands are important because this accepts the hypothesis that SNP exists in the DNA sample. If SNP existed in the sample, then the DNA sequence was not cleaved because the restriction enzyme HpaII would not be able to recognize the base pair change and cleave the sequence. Single nucleotide polymorphism have become increasing important today to…

The first process developed was the Restriction Fragment Length Polymorphisms (RFLP) Method of DNA Profiling. RFLP required large quantities of intact DNA and was really only effective at showing that two samples were different, making its application limited. Short Tandem Repeat (STR) Typing replaced RFLP…

Microbiota are microscopic organisms such as viruses, bacteria, archaea, and eukaryotes that influence human health through a commensal interaction. Location of microbiota can be internal or external such as the gut, mouth, placenta, skin, and vagina. These microbes are classified into four major phyla: Firmicutes, Bacteriodetes, Actinobacteria, and Proteobacteria. Microorganisms in the Firmicute phylum are typically Gram positive bacteria, either rod-shaped or spherical and function as obligate…