Paper chromatography

Analytical Method Validation of Sylimarin The λmax of Sylimarin in ethyl alcohol and PBS was found to be 286 nm and 322nm, respectively. The drug follows linearity in the concentration range 2–20 μg/ml with a correlation coefficient value of 0.998 and 0.999 for in ethyl alcohol and PBS, respectively. The accuracy of the method was checked by recovery. The % recovery was found to be in the range of 99.54– 100.98% in ethyl alcohol and 98.94– 101.4% in PBS, respectively. The precision of the…

Development and evaluation of an antimicrobial formulation containing the essential oil of Thymus fontanesii Boiss. & Reut . Mebarki Noudjoub , Nabiev Mohamed2, Chader Henni3, Ziane Henifa4, Fazouane Fethia1 1 Food Technology Research Laboratory, FSI, M’hamed Bougara University, Boumerdès 35000, Algeria 2 Department of Chemical and Pharmaceutical Process Engineering, FHC, M’hamed Bougara University, Boumerdès 35000, Algeria 3 National Laboratory of Control of Pharmaceuticals Products (NLCPP)…

The concentration of CDCL and CSER is obtained by solving equation (1) and (2) CX = A1 / ax1 ………………..... (1) CY = [A2 – (ax2 × CX)] / ay2…...(2) Where,  A1 and A2are absorbance of mixture at λ1 and λ2respectively.  ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,  ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,  CXandCYare concentrations of DCL and SER, respectively. Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4)…

Column Chromatography is considered one of the main techniques of separation in addition to SDS-PAGE and gel electrophoresis. This method can be useful in both a small scale as in a laboratory experiment as well as in a larger scale at the industrial level. It is also applied in different fields ranging from biology and biochemistry to microbiology and medicine. Column chromatography is also referred to as gel filtration or size exclusion chromatography among many other terminologies and it is…

Chromatography Abstract: The purpose of this lab is to use chromatography to separate and purify organic compounds. There are many different types of chromatography but most depend on the principle of adsorption. The two important components of chromatography are the adsorbent and the eluent. Adsorbents are usually solid materials that will attract and adsorb the materials to be separated. The eluent is the solvent, which carries the materials to be separated through the adsorbent. After being…

Thin-Layer Chromatography Lab 11/12/14 Beginning Question To what extent does the polarity of a substance affect the distance it travels during TLC using water as the solvent? Safety Concerns The substances used during the TLC process could possibly be dangerous, so it would be best to avoid getting them on the hands. If one were to accidentally spill the water used in the process onto the floor, a slipping hazard could occur. Data Dye Solvent Front (cm) Spot (cm) R…

polarity of substances using the principles of chromatography. Three substances were tested using a thin layer chromatography. The column chromatography tested a mixture of two substance which were separated as a result of the chromatography. The results for both the thin layer and column chromatography supported the hypothesis. Therefore, chromatography is an effective tool that can be used to determine relative polarities. INTRODUCTION: Chromatography is a laboratory procedure that operates…

Task 3 P1 When using thin layer chromatography (TLC) to test for purity of aspirin it is a quick and simple method. Firstly when using the TLC method you need to have a TLC sheet and mark on the sheet 0.5cm up on the sheet and draw a line, make sure you don’t press too hard on the paper as this will disrupt the absorbent on the paper. You need to make sure the samples have been marked on the paper so you don’t get mixed up and leave a gap between the aspirin samples so they don’t run together…

this purpose is called chromatography. 1 There are many different types of chromatography, including gas-liquid, paper, column and so on. 1 Chromatography differentiates by allowing the differences in polarity, vapor pressure, solubility and so on of different substances to be observed. 1 In our endeavor, we will be using specific types of chromatography, termed Thin-Layer Chromatography (TLC) and column chromatography. Essentially, TLC is an adsorption aspect of chromatography that uses a polar…

cell such as hair, skin, or plant cells all have DNA. Plenty of research and experimentation went into solving the structure of DNA and the invention of paper chromatography allowed Erwin Chargaff (1905-2002) to make a contribution in establishing the structure of DNA and how it transmitted genetic information. In 1950 he used paper chromatography to separate organic bases and measure the amount of each base in order to determine the ratio of the bases adenine-thymine (A-T) and…