Microbiology

viruses and fungus. This report will focus on food preservation which has been an issue for mankind ever since we started to package food and not eat it straight after we prepared it. The spread of disease is crucial in all aspects of microbiology and aseptic techniques are now followed by medical…

Application of Biofloc technology in aquaculture systems; a viewpoint Anjali P. Thilakan1*,Manju Lekshmi. N2.,Sreekanth.G.B2, N. P. Singh2 1Central Institute of Fisheries Education, Andheri (W), Mumbai 2ICAR Central Coastal Agricultural Research Institute, Ela, Old Goa . Abstract Bioflocs are aggregates (flocs) of algae, bacteria, proto¬zoans, and other kinds of particulate organic matter such as feces and uneaten feed and it normally contains 35-40% of protein. Biofloc technology ensures…

Body Bacteria Exploring the Skin’s Microbial Metropolis by Allison Maclachla, Personally I found this article very engaging yet descriptive. Especially when she explained that not all bacteria are bad for you, and then continues to state some are very helpful. For instance she touches base on Staphylococcus epidermis, and how it shields us by taking up the space that Staphylococcus aureus would otherwise conquer. It’s intriguing to now know that acne (Acne vulgaris) the most common skin…

Table 1 shows the all the isolates that produced a zone of inhibition for the ESKAPE pathogen, Staphylococcus aureus. In total, twenty six isolates were tested against Staphylococcus aureus. However, only eight showed a zone of inhibition. The size of the zones varied for each microorganism. Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and…

Gram + Cocci To identify the Gram + cocci I first performed a catalyst test which produced oxygen bubbles and indicated a positive catalase result. The next test I preformed was a mannitol salt test using a mannitol-salt agar plate and the results showed positive fermentation turning the plate yellow and also positive growth. This led me to conclusion that my unknown Gram + cocci was Staphylococcus aureus. Gram – Rod To identify the unknown Gram – rod I first performed a H2S production test…

Summary The purpose and goal of this project was to perform a number of tests in the lab and use a dichotomous key to eventually classify a Gram-positive cocci that we were given on March 25th. The key has seven tests to help you determine a certain bacteria, your tests will later on lead you down a certain side to contribute in defining the genus and species. All the following procedures were carried out on the gram positive cocci unknown in this order: Catalase, Oxidase, Nitrate Reduction,…

Ebola: a word that for many triggers a somewhat distant memory back to 2014 when the whole world was up in arms about it spreading and becoming a pandemic. This incident of outbreak in West Africa shows how far we have come as a world, technologically and medically, but also how far we still have to go. The severity of the outbreak was completely avoidable, however due to the disregard of certain factors not usually associated with disease, such as cultural practices, it became an epidemic. In…

ELVIS (Extraterrestrial Landing Vehicle Integrated Sampler) was placed on Mars to collect soil samples from the surface of the planet. The scientist’s goal, when ELVIS was launched, was to obtain soil samples from the planet that contained microbes distinguishable from those on Earth. However, when ELVIS returned home it was terribly damaged. The suit material and skin of ELVIS had degraded during the mission. Our goal as microbiologists was to determine if the microbes present in the soil…

4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…

CHAPTER-2 VARIABILITY AMONG THE ALTERNARIA BRASSICAE ISOLATES INTRODUCTION The chapter is mainly concerned with examining the variability among Alternaria brassicae isolates from the oilseed brassicas. Since, the oilseed brassicas are more prone to the fungal attack and thereby, resulting in reduced productivity of the oilseed. Therefore, the present investigation focuses on the studying the features such as morphological, pathological and molecular variability of Alternaria brassicae. The…