Membrane protein

Protein X has a pI of 5.9, Protein Y has a pI of 7.6, and Protein Z has a pI of 8.5. For each of the methods below, describe how you would go about purfying Protein Y from Proteins X and Z (provide a pH that you would use and why.) Anion exchange chromatography: In anion exchange chromatography negatively charged ions will bind to the column. This will leave positively charged ions flushed through the column. By picking a pH of 6.5, protein X will flush through the column while protein Y and Z…

What is Microneedling? Microneedling is a treatment that involves creating small wounds in the epidermis. This helps stimulate the production of new elastin and collagen. This treatment can help reduce the size of the pores. It can also wrinkles and improve skin texture. At Youth Reflections, micro-needling is performed along with platelet-rich plasma injections. It is called the RENEW procedure. PRP injections have been used in medicine for a long time in order to repair joint, tendon and…

Rituximab is an antibody that is primarily found on the surface of B cells in the immune system. It destroys B cells and thus is used to treat diseases that are characterized by B cell disorders e.g. overactive B cells, excessive numbers of B cells, or dysfunctional B cells. Rituximab use has been associated with increased risk of hepatitis B virus reactivation in patients who are negative for hepatitis B surface antigen (HBsAg) and positive for anti-hepatitis B core antibody (anti-HBc). The…

As a food science major with an interest in nutritional sciences the regulation of lipid synthesis would be a comprehensive topic that would allow me to develop my research abilities in a setting applicable to both my major and my interests. Using molecular biology and chemical processes, assisting in determining what mechanism is used to how phosphorylated PA phosphatase would allow me to learn both new lab techniques and new concepts in the fields of microbiology and biochemistry. My interests…

Enzymes are biological molecules macromolecules that catalyze chemical reactions. Enzymes are usually proteins; however, it has been discovered that some enzymes are RNA molecules. The molecules at the beginning of the process to which enzymes act upon are called substrates, which are converted to the products by the actions of the enzyme. Almost all metabolic processes require enzymes to catalyze reactions to a rate that is fast enough to sustain life. Enzymes increase the rate of the reaction…

We all want to look younger than we are and live as long as possible. Although its our genetic inheritance that gives us a head start, our diet also plays a vital part in helping us increase our lifespan and maintain beautiful youthful skin for longer. Today we at MindUnfold we reveal the 10 anti-aging foods that keep your skin at its finest. Avocado Youve probably heard that avocado makes you gain weight because of its high fat content. But perhaps you did not know that those fats are very…

Bio Lab- Denaturing Proteins Period B- Ms. Dann October 8, 2014 Nick D’Ambrosio, Matt Hermann, Carly Pruitt Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to…

Difference and Similarities of Lantus and Levemir Lantus and Levemir are the type of long-acting insulin which is injectable and used in the treatment of diabetes. They can be used by both children and adults. Human insulin is made up of amino acid chains, A and B. Insulin is produced naturally in the body by the pancreas. It converts tissues in the bloodstream into energy which we use daily. With no insulin in the torso, the sugar levels will become dangerously high and out of balance. Hence,…

The hormone insulin, made inside the pancreas with the help of beta cells, is released with each meal, to help the body store the blood glucose it gets from food (1). Your blood glucose level is an important level to shows how much sugar a person's body has in their blood, which in effect comes from the food that we eat. The insulin normally targets the liver because it’s the organ that removes glucose from the body turning it into glycogen.(2) There are many different types of insulin based off…

The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…