Gas chromatography

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    Analytical Method Validation of Sylimarin The λmax of Sylimarin in ethyl alcohol and PBS was found to be 286 nm and 322nm, respectively. The drug follows linearity in the concentration range 2–20 μg/ml with a correlation coefficient value of 0.998 and 0.999 for in ethyl alcohol and PBS, respectively. The accuracy of the method was checked by recovery. The % recovery was found to be in the range of 99.54– 100.98% in ethyl alcohol and 98.94– 101.4% in PBS, respectively. The precision of the…

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    Development and evaluation of an antimicrobial formulation containing the essential oil of Thymus fontanesii Boiss. & Reut . Mebarki Noudjoub , Nabiev Mohamed2, Chader Henni3, Ziane Henifa4, Fazouane Fethia1 1 Food Technology Research Laboratory, FSI, M’hamed Bougara University, Boumerdès 35000, Algeria 2 Department of Chemical and Pharmaceutical Process Engineering, FHC, M’hamed Bougara University, Boumerdès 35000, Algeria 3 National Laboratory of Control of Pharmaceuticals Products (NLCPP)…

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    Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

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    The concentration of CDCL and CSER is obtained by solving equation (1) and (2) CX = A1 / ax1 ………………..... (1) CY = [A2 – (ax2 × CX)] / ay2…...(2) Where,  A1 and A2are absorbance of mixture at λ1 and λ2respectively.  ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,  ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,  CXandCYare concentrations of DCL and SER, respectively. Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4)…

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    Column Chromatography is considered one of the main techniques of separation in addition to SDS-PAGE and gel electrophoresis. This method can be useful in both a small scale as in a laboratory experiment as well as in a larger scale at the industrial level. It is also applied in different fields ranging from biology and biochemistry to microbiology and medicine. Column chromatography is also referred to as gel filtration or size exclusion chromatography among many other terminologies and it is…

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    The data show that in separating the sample mix using size-exclusion chromatography, or gel filtration chromatography, the hemoglobin was able to be separated from the vitamin B12 suggesting that the data are consistent with the hypothesis. This is evident in the data since the concentration of protein decreased with each collected fraction after the first fraction that collected the protein, fraction 2, and leveled off. This is shown by the decreasing absorption measurements in Table 3. The…

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    During this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In…

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    Polyurethane Case Study

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    Introduction 1.1 Polyurethane polyurethanes were first developed by Otto Bayer and coworkers in 1937. Shortly thereafter, Schollenberger and coworkers used formula modification to prepare elastomeric polyurethane products from Bayer's products that were like nylon or other engineering plastics in physical characteristics. PUs are widely used in coatings, flexible and rigid foams, elastomers, and composites. Reasons for the use of polyurethane coatings include: high performance characteristics…

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    The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate…

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    Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

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