Enterococcus

The esp gene, associated with the surface protein of Enterococcus faecium, was used to detect the presence of this pathogen in the bay. The detection of esp was compared with the level of fecal indicator bacteria which includes Enterococci, fecal coliforms and Escherichia Coli. The times in which contamination…

The genera Streptococcus and Enterococcus both consist of Gram-positive cocci that are typically arranged in pairs or chains during growth.5 These bacteria are non-spore forming, facultative anaerobes.1, 5,6 While some are important members of the normal human microbiota, others are pathogenic and cause human diseases.1, 6 Thus far, the classification of these bacteria into major categories has been based on: 1. Serologic specificity of the cell wall group antigens (Lancefield antigens) and…

After getting a good gram stain the unknown chart were referred to in order to choose between appropriate biochemical tests. Referring to the unknown chart the three possible bacteria were Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis. The first biochemical test was to preform the Urea test. A sample of the gram-positive cocci colony was taken out which then was transferred into a Urea broth to test for Urease. This was incubated at 37 degrees Celsius for 48 hours.…

aureus), Acinetobacter calcoaceticus-baumannii complex, Pseudomonas aeruginosa, klebsiella pneumonia , Escherichia coli ( E.coli), Enterococcus, Burkholderia, Enterobacter, Proteus, coagulase negative staphylococci ( S.epidermidis, S. saprophytic). Although most of gram–ve bacilli can’t remain in dry surfaces and sensitive to common disinfectant, Acinetobacter calcoaceticus-baumannii complex…

exogenously attained, this leads to its resistance to the strongest antibiotics or sometimes all antibiotics. This is a large concern in hospital environments and with people with low immune systems since they are more susceptible to infection. Enterococcus faecalis usually inbabits the gastrointestinal tract in humans and animals. It can also be found in food, water, insects, birds and plants (Clauss). Typically it is considered a commensal bacterium because it benefits from living…

Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Carbohydrate Utilization: Materials: - Phenol red lactose Broth - Phenol Red Sucrose Broth - Unknown organism # 40 - Inoculating tool Procedure: Phenol red lactose and Phenol Sucrose were cultures used to identify fermentation of carbohydrates for the unknown organism #40. Phenol red is a ph. indicator as the inverted Durham tube indicates gas production…

Purpose To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods 1. One test that was used to discover the identity of the given bacterial culture was the carbohydrate utilization tests. This specific set of tests involved two broths, namely phenol red lactose and phenol red sucrose, and a Durham tube placed upside down in the broth tubes. These broths can be broken down by fermentation processes,…

to avoid complication along with envenomation. A very few microbiological data available on snakebite generated wound infection, a wide range of microorganism suggested including predominant aerobes viz. coagulase negative Staphylococcus aureus, Enterococcus, Enterobacteriaceae family species and anaerobes including Clostridium species. In light of the defined organisms, many authors had driven the work to find out the actual micro biota of snakes in many parts of the world. In view of the…

Abstract: Objective: To evaluate the ability of triple Taqman probe real-time PCR to the detection and differentiation of vanA, vanB and vanM genotypes of Vancomycin-resistant enterococci (VRE). METHODS: Choosing the culture method and PCR-sequencing assay as the reference standard，respectively, a total of 254 VRE and 90 strains of no-VRE were conducted by triple Taqman probe real-time PCR collected from 2005 to 2015 in China. Results: When detect the VRE strains, the sensitivity, specificity,…

and differential medium. The bile that was in the medium was selective for streptococci and enterococci and inhibited the growth of Gram-positive staphylococci (Atlas & Snyder, 2015). The test differentiated between Streptococcus bovis group and Enterococcus from other streptococci based on the bacteria’s ability to hydrolyze esculin in the presence of bile. When esculin is hydrolyzed the byproduct reacts with ferric citrate and forms a dark precipitate on the medium (Atlas & Snyder, 2015). A…